| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kenshi Faculty of Med., KYUSHU UNIVERSITY, Prof., 遺伝実験情報施設, 教授 (00019671)
TAMIYA Sadafumi School of Health Sciences, KYUSHU UNIVERSITY, Ass.Prof., 医療短期大学, 講師 (60284486)
ODA Yoshinao Faculty of Med., KYUSHU UNIVERSITY, Ass.Prof., 大学院・医学研究院, 講師 (70291515)
FUKUDA Toshiro Funuoka Red Cross Hospital, Chief, 部長(研究職)
KAWAUCHI Shigeto Yamaguchi Univ.Fac.of Med., Ass.Prof., 医学部・第二病理, 講師
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| Research Abstract |
We assessed the biological character of bone and soft tissue tumors by molecular biology technique in addition to clinico-pathologic analysis.
1. Malignant transformation : 1) Neurofibroma to malignant peripheral nerve sheath tumor ; abnormal expression of p53 protein, 2) Dermatofibrosarcoma protuberans to fibrosarcoma ; chromosomal abnormality.
2. Dedifferentiated chondrosarcoma and dedifferentiated liposarcoma characterized by high grade malignancy : H-ras mutation
3. Biological behavior in sarcomas
1) Leiomyosarcoma : p53 mutation in 4 dead cases among favorable superficial type. Using Real-Time Semi-quantitative RT-PCR, an increasing level of p53 mRNA associated with an overexpression of p53 protein. 2) Synovial sarcoma : Unfavorable prognostic factors (i) high AJC stage, (ii) large tumor size, (iii) co-expression of HGF/c-MET, (iv) mutation of p53, MDM2, H-ras, beta-catenin. 3) Malignant rhabdoid tumor (i) Immunoexpression of cytokeratin 8 and 18, (ii) Subcellular distribution of cytokeratin and vimentin in rhabdoid cell by three-dimensional imaging, (iii) Genomic abnormality of vimentin and cytokeratin, (iv) Unfavorable prognostic factors : abnormal expression of beta-catenin, p53, hSNF5/INI1 4)Osteosarcoma : Significant nm23, c-MET in metastasis and tumor progression.
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