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1999 Fiscal Year Final Research Report Summary

The Mechanism of Stimulus Secretion Coupling in the Endocrine Tumors

Research Project

Project/Area Number 09470248
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ONODERA Hisashi  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (50240825)

Co-Investigator(Kenkyū-buntansha) INOUE Kazutomo  Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (90168435)
HOSOTANI Ryo  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 助教授 (00139908)
IMAMURA Masayuki  Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (00108995)
SHIMADA Yutaka  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (30216072)
Project Period (FY) 1997 – 1999
Keywordspancreas / endocrine tumor / calcium / secretin / calcium-sensing receptor
Research Abstract

1) We investigated the mechanism of hormone release from the pancreatic endocrine tumors. We already found that the insulin release from insulinoma cells are induced by the elevation of intracellular calcium concentration ([CaィイD12+ィエD1]i) and this [CaィイD12+ィエD1]i elevation is stimulated by the increase of extracellular calcium concentration ([CaィイD12+ィエD1]o). Therefore we paid attention and examined the expression of calcium-sensing receptor (CaR) in the insulinoma. Using electrophysioligical study, Southern blot analysis and immuno-histochemistry, it was proved that the CaR is expressed in insulinoma, gastrinoma and also in the normal islets. However, the responses of the CaR to calcium in endocrine tumors were different from the responses in the normal islets. We think this difference between the endocrine tumors and the normal islets plays an important role in the abnormality of hormone release in the endocrine tumors. Now, we further examines the function of CaR in the endocrine tumors.
2) It was confirmed that the human normal antral G cells and gastrinoma express the secretin receptor by RT-PCR. We are presently making the anti-secretin-receptor antibody to investigate the distribution of secretin receptor in the pancreatic endocrine tumors.
3) Concerning the culture and passage of endocrine tumor cells, in preliminary study, we succeeded in long term culture of normal islet cells using the chitosan mesh.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Hosotani,Ryo: "Surgical management of endocrine tumor of the pancreas in Japan"Journal of Hepato-Biliary-Pancreatic Surgery. 4 ・ 3. 295-303 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小切匡史: "消化管ホルモン産出腫瘍"ホルモンと臨床. 45・10. 989-996 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imamura,Masayuki: "Progress in the field of pancreatic endocrine tumors in Japan"Pancreas. 16. 379-384 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imamura,Masayuki et al.: "Rationale of so-called extended resection for pancreatic invasive ductal carcinoma"Digestion. 60. 126-129 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 細谷亮: "肝臓手術の新しいテクニック:SASItestとIOStestによるガストリノーマ根治術"外科. 61. 31-36 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosotani, Ryo: "Surgical Management of Endocrine Tumor of the Pancreas in Japan"Journal of Hepato-Biliary-Pancreatic Surgery. 4/3. 295-303 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura, Masayuki: "Progress in the Field of Pancreatic Endocrine Tumors in Japan"Pancreas. 16. 379-384 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura, Masayuki: "Rationale of So-called Extended Resection for Pancreatic Invasive Ductal Carcinoma"Digestion. 60 (Supple 1). 31-36 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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