• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Evaluation of focal cerebral ischemia by NMR and histochemistry.

Research Project

Project/Area Number 09470327
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ARAI Toshiyuki  Kyoto University, Medicine, Associated Professor, 医学研究科, 助教授 (80175950)

Co-Investigator(Kenkyū-buntansha) INUBUSHI Toshiro  Shiga University of Medical Science, Professor, 分子神経生物学研究センター, 教授 (20213142)
Project Period (FY) 1997 – 1998
Keywordsfocal cerebral ischemia / rat / global brain ischemia / gerbil / nuclear magnetic resonance / lactate / immunohistochemistry / interleukin-1 receptor antagonist
Research Abstract

Effects of neroprotective drugs on cerebral ischemia were evaluated in animals using nuclear magnetic resonance (NMR) and immunohistochemical analyses.
1) Focal cerebral ischemia was introduced in spontaneously hypertensive rats (STIR) using a method of intraluminal vascular occlusion. Then, lactate produced in the damaged area was detected by ^1H NMR.spectroscopy. The increase in lactate was larger in transient ischemia than that in permanent one. The increase in lactate in transient ischemia was attenuated by alpha-phenyl-N-tert- butyl nitrone (PBN), a superoxide scavenger.
2) Expression of interleukin-1 receptor antagonist (IL- lra) was investigated in rat brain following transient focal cerebral ischemia induced by 30 min of middle cerebral artery occlusion. The expression of IL-lra was observed on the margin of the neuronal damage 1 day after operation, which was enhanced 4 days after operation and withdrawn 7 days after operation.
3) Neuroprotective effects of PBN and oxypurinol, a xanthine oxidase inhibitor, were examined in gerbil global brain .ischemia. Ischemic neuronal damage were evaluated one week after a 3.5 mm bilateral carotid artery occlusion by a histochemical analysis (cresyl violet staining). PBN administered 30 min after ischemia partially attenuated the neuronal damage, while oxypurinol did not.
These results indicated that the drug effects on focal cerebral ischemia were precisely evaluated using NMR and immunohistochemical analyses.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Arai T, Nakao S, et al.: "Measurement of local cerebral blood flow by magnetic resonance imaging : In vivo autoradiographic strategy using 17O-labeled water." Brain Res Bull. 45. 451-456 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai T, Mori H, et al.: "Auto-oxideation of 5, 6, 7, 8-tetrahydroneopterin." Pteridines. 9. 26-28 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori H, Arai T, et al.: "Neuroprotective effects of pterin-6-aldehyde in gerbil global brain ischemia : comparison with those of α-phenyl-N-tert-butyl nitrone" Neurosci Lett. 241. 99-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii H, Arai T, et al.: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses." J Cereh Blood Flow Metah. 18. 931-934 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai T, Mori H, et al.: "Oxypurinol, a xanthine oxidase inhibitor and a superoxide scavenger, did tno attenuate ischemic neuronal damage in gerbil" Life Sei. 63. PL107-112 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai T,Nakao S,Morikawa S,Inubushi T,Yokoi T,Shimizu K,Mori K: "Measurement of local cerebral blood flow by magnetic resonance imaging : In vivo autoradiographic strategy using ^<17>O-labeled water" Brain Res Bull. 45. 451-456 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai T,Mori H,Ishii H,Suzuki T,Kojima S,Mori K: "Auto-oxidation of 5,6,7,8-tetrahydroneopterin" Pteridines. 9. 26-28 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori H,Arai T,Ishii H,Adachi T,Endo N,Makino K,Mori K: "Neuroprotective effects of pterin-6-aldehyde in gerbil global brain ischemia : comparison with those of alpha-phenyl-N-tert butyl nitrone" Neurosci Lett. 241. 99-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii H,Arai T,Morikawa S,Inubushi T,Tooyama I,Kimira H,Mori K: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses." J Cereb Blood Flow Metab. 18. 931-934 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai T,Mori H,Ishii H,Adachi T,Endo N,Makino K,Mori K: "Oxypurinol, a xanthine oxidase inhibitor and a superoxide scavenger, did not attenuate ischemic neuronal damage in gerbils." Life Sci. 63. PL107-112 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi