1998 Fiscal Year Final Research Report Summary
Development of electrodes modified with biocompatible molecules for electrochemical imaging of chemicals in biological samples
| Project/Area Number |
09470494
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
OHMORI Hidenobu Osaka University, Graduate School of Pharmaceutical Sciences/Professor, 薬学研究科, 教授 (90028845)
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| Project Period (FY) |
1997 – 1998
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| Keywords | modified electrode / biocompatibility / protein-adsorption / HPLC analysis / biological compound / electrochemical analysis / glassy carbon electrode |
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| Research Abstract |
This research has been performed, being directed to development of electrodes modified with biocompatible molecules available for electrochemical imaging of chemicals in biological samples, and has afforded the following interesting results.
1. Evaluation of electrochemical and physical performance of glassy carbon (GC) electrodes anodized in HO(CH^2CH^2O)^n R(n=l-4, R=H or CH^3) has been demonstrated that a GC electrode anodized in HO(CH^2CH^2O)^3H (Electrode 1) has the best applicability to electrochemical analysis of biological samples. By HPLC with Electrode 1, analysis of acetaminophen in urine samples is carried out with desirable sensitivity and high reproducibility without any pretreatment for removal of proteins.
2. Utilizing a GC electrode anodized successively in H20 and HO(CH^2CH^2O)^3H (Electrode 2) instead of Electrode 1, HPLC analysis of acetaminophen in urine samples can be conducted with a higher sensitivity. Electrode 2 has proved to have good electrochemical performanc
… More
e toward various biologically important compounds including several catecholamines, suggesting that HPLC with Electrode 2 will find a useful utility for analysis of the compounds in biological samples.
3. Coating with a polymer anodically formed from m-HOC6H4O(CH2CR2O)4CH3 has shown to serve as a tool for modification of surfaces of Au as well as GC electrodes with biocompatible molecules.
4. It was demonstrated that anodization in HO(CH^2CH^2O)^3H containing HOCH^2CH^2SO^3Na affords a GC electrode (Electrode 3) with -O(CH^2CH^2O)^2CH^2CO^2H group on its surface. Electrode 3 exhibits interesting electrochemical performance with a high selectivity toward cationic compounds including various biologically important catecholamines due to the anionic functional groups on the surface.
5. Using carboxyl groups on Electrode 3, 4-amino-2,2,6,6-tetramethylpiperidiny1-1-oxy radical (4-amino-TEMPO) or 3,4-dihydroxybeazylamine (3,4-DHBA) can be easily immobilized on the surface of a GC electrode. HPLC with the electrode having immobilized 4-amino-TEMPO is useful for analysis of sugars such as glucose at desirably low applied potential. The electrode with immobilized 3A-DHBA serves as an electrochemical mediator for NADH oxidation in the presence of Mg2^<2+>. Less
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Research Products
(6 results)
