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1999 Fiscal Year Final Research Report Summary

Determination and differentiation of heamtopoietic stem cell toward T, B and myeloid cell lineages

Research Project

Project/Area Number 09557029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Immunology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KATSURA Yoshimoto  Institute for Frontier Medical Science Professor, 再生医科研究所, 教授 (90027095)

Co-Investigator(Kenkyū-buntansha) TAMURA Masahiko  Chugai Pharmaceutical Co., LTD. Researcher, 創薬研究所, 主任研究員
Project Period (FY) 1997 – 1999
Keywordshematopoietic stem cell / differentiation / commitment / T cell / thymus / AGM region / lineage committed progenitor
Research Abstract

By using the multilineage progenitor (MLP) assay, which is able to determine the developmental capability of hematopoietic progenitors toward myeloid (M), T and B cell lineages, we have identified various types of progenitors in murine fetal liver (FL). The progenitor types detected were multipotent progenitors (p-MTB), bipotent progenitors generating M and T cells (p-MT) or M and B cells (p-MB), and unipotent progenitors generating M(p-M), T(p-T) or B(p-B) cells. Since p-TB type was defective, p-T and p-B were thought to be derived from p-MTB via p-MT and p-MB, respectively. These findings provided an opportunity to reconsider the process of hematopoiesis; it is improbable that the lineage commitment progresses stochastically, but it should progress deterministically. It was further shown that p-T emerge ontogenically earlier than p-B in FL, and moreover that all six types of progenitors are already produced in the aorta-gonad-mesonephros (AGM) region of day 10 fetuses. Although the p-MTB in AGM region do not have long-term reconstitution (LTR) activity, these p-MTB was found to acquire the LTR activity by culturing with a stromal cell line. Thymic earliest p-T are still multipotent in that they are able to generate dendritic cell (DC) and NK cells. These DC and NK potentials are release with the progress of intrathymic developmental stages.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kawamoto,H. et al.: "Emergence of T cell lineage restricted progenitors at the earliest stage of hematopoiesis in the murine fetal liver"Journal of Immunology. 162 5. 2725-2731 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Van Ewijk,W., et al.: "Thymic miroenvironments, 3-D versus 2-D?"Seminars in Immunology. 11 1. 57-64 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawabata,K. et al.: "A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution"Proc.Natl. Acad. Sci. USA. 96 10. 5663-5667 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohmura,K. et al.: "Emergence of T,B and myeloid lineage commitment as well as multipotent hematopoietic progenitors in the AGM region of day 10 fetuses of the mouse"Jounral of Immunology. 163 9. 4788-4795 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikawa,T.et al.: "Commitment of common T/NK progenitors to unipotent T and NK progenitor in the murine fetal thymus revealed by a single progentitor assay"Journal of Experimental Medicine. 190 11. 1617-1625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto,H. et al.: "T cell progenitors emerge earlier than B cell progenitors in the murine fetal liver"Immunity. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto, H. et al: "Emergence of T cell lineage lineage restricted progenitors at the earliest stage of hemaopiesis in the murine fetal liver"Journal of Immunology. 162(5). 2725-2731 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] van Ewijk, W., et al: "Thymic miroenvironments, 3-D versus 2-D ?"Seminars in Immunology. 11(1). 57-64 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawabata, K. et al: "A cell-autonomous requirement for CXCR4 in long-term USA lymphoid and myeloid reconstitution"Proc, Natl. Acad. Sci. 96(10). 5663-5667 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohmura, K. et al: "Emergence of T, B and myeloid lineage commitment as well as multipotent hematopoietic progenitors in the AGM region of day 10 fetuses of the mouse"Jounral of Immunology. 163(9). 4788-4795 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikawa, T.et al: "Commitment of common T/NK progemotprs tp unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay"Journal of Experimental Medicine. 190(11). 1617-1625 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, H. et al: "T cell progenitors emerge earlier than B cell progenitors in the murine fetal liver"Immunity. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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