1998 Fiscal Year Final Research Report Summary
Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine"
| Project/Area Number |
09557202
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
医薬分子機能学
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| Research Institution | Osaka University |
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Principal Investigator |
TSUTSUMI Yasuo School of Pharmaceutical Sciences Osaka University Research Associate, 薬学研究科, 助手 (50263306)
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| Co-Investigator(Kenkyū-buntansha) |
MAYUMI Tadanori School of Pharmaceutical Sciences Professor, 薬学研究科, 教授 (00098485)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Cytomedicine / APA-microcapsule / Immunoisolation / Drug Delivery System |
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| Research Abstract |
Microencapsulation of living cells or tissues has been proposed to prevent their immune destruction following transplantation. In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. The APA membrane inhibited the activation of the host's cellular immune response, but did not prevent the production of cytotoxic antibodies against entrapped SK2 cells following allogeneic transplantation. However, the APA-SK2 cells remained vital in SK2 cell-immunized mice as well as in intact mice. We considered that complement regulatory factors which were present on cell membrane and had species-specific restriction blocked the complement-mediated cell lysis on allogeneic transplantation, since APA-SK2 cells were destroyed by rabbit anti-SK2 cell antiserum. Our results demonstrated that APA membrane could inhibit cell-cell contact between entrapped cells and the host's lymphocytes, but could not completely protect the entrapped cells from xenogeneic hamoral immunity.
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[Publications] Okada N., Miyamoto H., Kaneda Y., Yamamoto Y., Katsume A., Saito H., Yorozu K., Ueda O., Tsutsumi Y., Nakagawa S., Oshugi Y., and Mayumi T.: "Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine" ; Therapeutic effect on IgG1 plasmacytosis and mesangio-proliferative glomerrulonephritis in the interleukin-6 transgenic mouse." J.Control.Release. 44. 195-200 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Okada N., Miyamoto H., Yoshioka T., Katsume A., Saito H., Yorozu K., Ueda O., Itoh N., Mizuguchi H., Nakagawa S., Oshugi Y., and Mayumi T.: "Cytomedical therapy for IgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly (L) lysine-alginate membrane." Biochem.Biophs.Acta.1360. 53-63 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Mizuguchi H., Nakagawa T., Toyosawa S., Nakanishi M., Imazu S., Nakanishi T., Tsutsumi Y., Nakagawa S., Hayakawa T., Ijuhin N., and Mayumi T.: "Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes in to the artery that leads to tumors." Cancer Res.58. 5725-5730 (1998)
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「研究成果報告書概要(欧文)」より
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