1998 Fiscal Year Final Research Report Summary
Epstein-Barr virus latent membrane protein-1 oncogene deletions in Hodgkin's disease and gastric carcinoma in Japan and Nepal.
| Project/Area Number |
09670177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | SCHOOL OF MEDICINE, CHIBA UNIVERSITY |
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Principal Investigator |
ISHII Genichiro CHIBA UNIVERSITY,SCHOOL OF MEDICINE.Assistant, 医学部, 助手 (00270869)
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| Co-Investigator(Kenkyū-buntansha) |
HARIGAYA Kenichi CHIBA UNIVERSITY,SCHOOL OF MESICINE.Proffesor, 医学部, 教授 (40101894)
TAMARU Junichi CHIBA UNIVERSITY,SCHOOL OF MESICINE.Lecturer, 医学部, 講師 (30188429)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Epstein-Barr virus / Latent membrane protein-1 / Hodgkin's disease / Gastric carcinoma |
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| Research Abstract |
The presence of 30-bp deletions within the carboxy terminal end of the LMP-1 oncogene of EBV has been reported in EBV-aasociated neolasms. We analysed the 30-bp deletion of the LMP-1 gene in Hodgkin's disease and gastric carcinoma in Japan and Nepal. 35% of Hodgkin's cells in Japan showed positive reaction for LMP-1. Direct sequence analysis of the PCR products of carboxy terminal end of the LMP-1 oncogene revealed identical 30-bp deletion in 5 of 6 samples of EBV-associated Hodgkin's disease. In EBV-associated gastric carcinoma in Japan, this deletion was found in 2 of 3 samples.
On the other hand, 100% of Hodgkin's cells showed positive reaction for LMP-1 in Nepal. Identical 30-bp deletion was observed in 4 of 8 samples.
Furthermore we examined the proliferating activity and P53 expression of Hodgkin's cells with and without 30-bp deletion. However proliferating activity and P53 expression was almost similar between these two groups.
Our results suggest that the 30-bp deletion mutant is widely spread in Japan and Nepal and is not implicated in EBV-associated neolasms.
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