1999 Fiscal Year Final Research Report Summary
Regulation of immune response in hole body and intestinal mucosa by oral administration of staphylococcal enterotoxin B (SEB)
| Project/Area Number |
09670542
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TSUJIKAWA Tomoyuki Shiga University of Medical Science, Department of Internal Medicine, Instructor, 医学部, 助手 (80273407)
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| Project Period (FY) |
1997 – 1999
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| Keywords | SEB / DSS colitis / TCR α KO mice / Vβ8T-cell / oral torelance |
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| Research Abstract |
We have been elucidated that oral SEB administration prevented the onset of DSS induced colitis, and influence of clonal deletion designated by Vβ8T -cells. We evaluated the effect of oral SEB administration on TCR α KO mice. (Experiment 1) Simultaneous administration of SEB ameliorated DSS induced colitis on TCR α KO mice, following prolonged survival time. Although serum Ig M of mice administered DSS increased more than that of control, the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells and CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells did not change with or without SEB oral administration. (Experiment 2) Although TCR α KO mice have been given SEB for 9 weeks, the incidence of colitis did not decrease and the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells also did not change. It was suggested that CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cell may influence the onset of the colitis on TCR α KO mice. We elucidated that preventive effect of SEB on DSS induced colitis of TCR α KO mice. Therefore, it is suggested that TCRαィイD1-ィエD1βィイD1+ィエD1 cells which express the Vβ region corresponding SEB may play an important role on the onset of DSS induced colitis on TCR α KO mice. However, it was difficult to induce oral tolerance by SEB under condition of continuing local inflammation such as colitis of TCR α KO mice.
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Research Products
(10 results)
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[Publications] Tsujikawa T, Ohta N, Nakamura T, Satoh J, Uda K, Ihara T, Okamoto T, Araki Y, Andoh A, Sasaki M, Fujiyama Y, Bamba T.: "Medium-chain triglycerides modulate ileitis induced by trinitrobenzene sulfonic acid."J Gastroenterol Hepatol. 14(12). 1166-1172 (1999)
Description
「研究成果報告書概要(欧文)」より
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