1999 Fiscal Year Final Research Report Summary
Functions of gap-junction in peripheral vascular smooth muscle in heart failure
| Project/Area Number |
09670765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SATO Naoki Nippon Medical School, Medicine, Lecturer, 医学部, 講師 (70291721)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Heart Failure / Gap-junction / アドレナリン受容体 |
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| Research Abstract |
[Goal] To clarify the influences to adrenergic responses by the dysfunction of gap junction in mesenteric arteries, which are obtained from the beagle model with rapid pacing induced heart failure.
[Methods/Results/Discussion] Right ventricular rapid pacing at 260 bpm induced 60% reduction of inotropic responses of isoproterenol 5 weeks after pacing. We performed additional experiments to develop the techniques using pulmonary arteries of monocrotaline-induced pulmonary hypertension rats, as the function of gap junction is impaired in this model. Heptanol at the dose of 500 μM reduced the responses to phenylephrine (200nM) by 35%. However, the results were influenced by the temperature in the organ bath. We modified the system to steady the temperature in the bath. Organ bath chambers were maintained at 37±0.05℃. However, it was actually not consistent in the chamber, because it was continuously bubbled. Chist GJ et al.(ref 1) did not mention how the temperature was controlled. It took 3 years to set it up. We are planning to continue the present study using rapid pacing induced heart failure.
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