1999 Fiscal Year Final Research Report Summary
Physiological intrahepatic cholestasis by breast milk
| Project/Area Number |
09670808
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tottori University |
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Principal Investigator |
MURA Tatsuo Department of Pathological Biochemistry, Tottori University School of Medicine, Lecturer, 医学部, 講師 (80093631)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Yumi Department of Pediatrics, Tottori University School of Medicine, Assistant, 医学部・附属病院, 助手 (50243390)
OGURA Yoshio Department of Biochemistry, Tottori University School of Medicine, Assistant, 医学部, 助手 (00135849)
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| Project Period (FY) |
1997 – 1999
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| Keywords | biliary obstruction / sinusoidal bile salt transport protein / basolateral plasma membrane / bile salt-dependent flow / osmolarity / multi variate regression of bile flow / breast milk / intracellular cyclic AMP level |
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| Research Abstract |
Studies of bile flow physiology are hampered by the lack of techniques such as micropuncture, which might directly assess different components of bile flow. Indirect techniques such as linear regression analysis and measurement of canalicuar flow with markers, which are 14C-labelled bile acids, must be interpreted cautiously. In these studies, we have set out to examine the cause of the large range of effect that a single bile acid or in combination of breast milk can have on bile flow.
The following aspects of the present study appear to be interpretable with some confidence. The variable effect of bile acid is not accompanied by changes in bile osmolarlity. Second, a good correlation exists between the effect of bile salts on flow and the mass of electrolyte secreted in conjugation with bile salts. Finally, when breast milk addition can not reduce the effect of bile salt on flow, an action previously demonstrated by bile salt-associated electrolyte secretion is not reduced.
It seems reasonable to conclude that the variable effect of bile salt on bile flow is not due to generation of fluid of variable tonicity. The final conclusion is based on the fact that breast milk is substance which alters cellular electrolyte transport and has been used extensively in previous studies to do so. It raises intracellular cyclic AMP levels, which it is of the couple or electrogenic type. It is very unlikely that the effect secondary acting on the liver.
Breast milk is not a physiological regulator, but the fact that variations in the control rat study were spontaneous and unrelated to the time from surgery suggests that there are physiological regulators. Furthermore, these results suggest that hormones in breast milk may be the regulators, especially somatostatin.
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