1998 Fiscal Year Final Research Report Summary
Analysis of Fibrillogenesis for Inactivation of TGF-beta by Decorin
| Project/Area Number |
09670866
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Chiba University School of Medicine |
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Principal Investigator |
SHINKAI Hiroshi Chiba Univ., Sch.Med., Professor, 医学部, 教授 (90030957)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Hideji Chiba Univ., Sch.Med., Assistant, 医学部, 助手 (50282489)
KURODA Kei Chiba Univ., Sch.Med., Assistant, 医学部, 助手 (90225300)
HATAMOCHI Astushi Chiba Univ., Sch.Med., Associato Professor, 医学部, 助教授 (90172923)
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| Project Period (FY) |
1997 – 1998
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| Keywords | TGF-beta / interleukin-4 / hypertrophic scar / systemic sclerosis ; / skin fibrosis. |
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| Research Abstract |
Dermatopont in is a recently discovered extracellular matrix protein with proteoglycan and cell binding properties and is assumed to play important roles in cell-matrix interactions and matrix assembly. En this study we examined the expression of dermatopont in mRNA and protein in skin fibroblast cultures from patients with hypertrophic scar (HS) and patients with systemic sclerosis (SSc). Dermatopont in mRNA and protein levels were reduced in fibroblast cultures from HS lesional skin compared to fibroblasts from normal skin of the same HS patient. Fibroblast cultures from SSc patient involved skin also showed significant reduction of dermatopontin expression compared to normal skin fibroblasts from healthy individuals. We investigated further the effects of cytokines and matrix collagen on dermatopontin expression in normal cultured fibroblasts. Transforming growth factor-beta1 increased dermatopontin mRNA and protein levels, while interleukin-4 reduced dermatopontin expression. Substrate coated with type I collagen reduced dermatopontin mRNA levels, the reduction being more prominent in three-dimensional collagen matrices. Our results suggest that the decreased expression of dermatopontin is associated with the parhogenesis of fibrosis in HS and SSc, and that the effect of the cytokines and matrix collagen on dermatopont in may have important implications for tissue remodeling or fibrosis.
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