1998 Fiscal Year Final Research Report Summary
The composition and the pathological significance of C1q-binding immune complexes in MRL/lpr mice
| Project/Area Number |
09671152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
UWATOKO Shu University of Tokyo Health Service Center Lecturer, 保健管理センター, 講師 (30133078)
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| Project Period (FY) |
1997 – 1998
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| Keywords | systemic lupus erythematosus / MRL / lpr mice / immune complexes / autoantibodies / C1q / antiDNAantibodies / collagen-like region of C1q |
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| Research Abstract |
Our previous studies have shown that the majority of C1q-binding IgG in patients with systemic lupus erythematosus (SLE) is composed of autoantibodies to the collagen-like region of C1q. We also showed that C1q-binding IgG in MRL/l mice, in contrast to human SLE, is large-sized immune complexes containing antibodies to DNA.DNase I digestion of C1q-binding IgG failed to diminish C1q-binding activity, suggesting that other negatively-charged substances might be present as antigens in these C1q-binding immune complexes.
This study was undertaken to characterize the antigens in C1q-binding immune complexes in MRL/l mice. Laminin and collagens were tested by ELISA, and glycosaminoglycans (hyaluronic acid, chondroitin sulfate, heparin, heparan sulfate) were examined by the effects of enzyme-digestion (hyaluronidase, chondroitinase, etc.) on C1q-binding activities of these immune complexes. In addition, the effects of phospholipases were also investigated for possible involvement of lipids in C1q-binding activity. No evidence, however, was obtained for the presence of these substances in the C1q-binding immune complexes.
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[Publications] Seki, G., Yamada, H., Taniguchi, S., Uwatoko, S., Suzuki, K., Kurokawa, K.: "Mechanism of anion permeaation in the basolateral membrane of isolated rabbit renal proximal tubule S3 segment." Am. J.Physiol. 272. C837-846 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Seki, G., Yamada, H., Taniguchi, S., Uwatoko, S., Suzuki, K., Kurokawa, K.: "Mechanism of anion permeaation in the basolateral membrane of isolated rabbit renal proximal tubule S3 segment." Cell Physiol. 41.
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「研究成果報告書概要(欧文)」より
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[Publications] Okawa-Takatsuji, M., Aotsuka, S., Fujinami, M., Uwatoko, S., Kinoshita, M., Sumiya, M.: "Up-regulation of intercellular adhesion molecule-1(ICAM-1), endothelial leukocyte adhesion molecule-1(ELAM-1)and class II MHC molecules on pulmonary artery endothelial cells by antibodies against U1-ribonucleoprotein." Clin. Exp. Immunol.(in press).
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「研究成果報告書概要(欧文)」より
