1998 Fiscal Year Final Research Report Summary
Experimental study about efficacy of treatment for metastatic lung tumor using Matrix Metalloproteinasa Inhibitor An experimental study of pulmonary artery infusion with cisplatin for solitary pulmonary tumor model using rat colorectal adenocarcinoma cell
| Project/Area Number |
09671400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | KANSAI MEDICAL UNIVERSITY |
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Principal Investigator |
SAITO Yukihito Kansai Medical University, Medicine, Associate Professor, 医学部, 助教授 (50131438)
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| Co-Investigator(Kenkyū-buntansha) |
OMIYA Hideyasu Kansai Medical University, Medicine, Assistant Professor, 医学部, 助手 (70309219)
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| Project Period (FY) |
1997 – 1998
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| Keywords | pulmonary artery infusion / lung tumor / open lung injection |
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| Research Abstract |
Objectives : The purpose of this study is to assess an availability of the tumor model made by open lung injection for an experiment about metastatic lung tumor by confirmation of its blood supply, and to evaluate an efficacy of pulmonary artery infusion (PAI) with cisplatin in this model using rat colorectal adenocarcinoma (RCN-9) cell line.
Methods : Male F344 rats were used. In experiment 1, rat colorectal adenocarcinoma (RCN-9) cells were injected to the rat left lung on day 0. On day 10, tissue blood flow of the tumors before/after pulmonary arterial occlusion were measured. I experiment 2, RCN-9 cells were injected to the rat left lung, and no other treatment was performed in the control group. The other group was performed PAL with 0.1 mg of cisplatin on day 3. On day 10, all rats were sacrificed and measured the maximum cross sectional areas of the tumors. Results : In experiment 1, the tissue blood flow of the tumors was significantly decreased after the pulmonary. arterial occlusion. In experiment 2, the maximum cross sectional areas of the tumors in the PAI group were significantly lower than those in the control group.
Conclusion : The blood flow of the tumor is supplied from pulmonary artery, so this model seems to be useful for the experiment about metastatic lung tumor. The tumor sizes were significantly reduced with PAI in this model. The local chemotherapy with PAI could be an effective choice of therapy for metastatic lung tumor from colorectal adenocarcinoma.
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