1999 Fiscal Year Final Research Report Summary
Basic Research for Hyperthermia in Prostate Cancer
| Project/Area Number |
09671648
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
DEGUCHI Nobuhiro Saitama Medical School, Professor, 医学部, 教授 (90118977)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Munehisa Saitama Medical School, Assistant Professor, 医学部, 助教授 (60167286)
OHIGASHI Takashi Keio University School of Medicine, Instructor, 医学部, 講師 (80185371)
NAKANOMA Takashi Saitama Medical School, Assistant, 医学部, 助手 (50198173)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Heat / Prostate cancer / Cell cycle / Heat shock protein 70 / Apoptosis / Quercetin |
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| Research Abstract |
It has been demonstrated that prostate cancer cells are relatively sensitive to heat stress. Heat treatment at 43℃ inhibited the cell growth of three different prostatic cell lines, LNcap, JCA-1, and PC-3. Heat treatment at 43℃ increased the expression of heat shock protein 70 (hsp70) in prostate cancer cells, leading to cell death through apoptosis. Hsp70 is known to be a protein that protect cells against heat damage. Cells with lower levels of hsp70 have been shown to have a higher sensitivity to heat stress. Therefore down-regulation of hsp70 is expected to enhance heat-induced inhibitory effects on cell growth. Quercetin has been reported to be an agent that inhibits hsp70 expression. The cells were treated with quercetin and/or heat. Treatment with quercetin alone resulted in an apparent decrement of hsp70-positive cells and an increment of subG1 cells in JCA-1 and LNcap cells. Quercetin inhibited an increment of hsp70 expression after heat treatment and increased in the number of subG1 cells with lower levels of hsp70 in JCA-1 and LNcap cells. Quercetin was found to enhance the heat-induced inhibitory effects on cell growth and heat-induced apoptosis in both JCA-1 and LNcap cells. These results suggest that quercetin may enhance heat-induced cytotoxicity in prostate cancer cell lines through the inhibition of hsp70 production.
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