1999 Fiscal Year Final Research Report Summary

MATRIX METALLOPROTEINASE IN TRABECULAR MESHYWORK

Research Project

Project/Area Number	09671807
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Ophthalmology
Research Institution	KUMAMOTO UNIVERSITY SCHOOL OF MEDICINE
Principal Investigator	MIYAGAWA Shin-ichi KUMAMOTO UNIVERSITY, OPHTHALMOLOGY, ASSISTANT PROFESSOR, 医学部, 助手 (10260738)
Co-Investigator(Kenkyū-buntansha)	FUKUSHIMA Mikiko KUMAMOTO UNIVERSITY, OPHTHALMOLOGY, ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (10284770) MATSUMOTO Koki KUMAMOTO UNIVERSITY, OPHTHALMOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (70173896) NEGI Akira KUMAMOTO UNIVERSITY, OPHTHALMOLOGY, PROFESSOR, 医学部, 教授 (00189359)
Project Period (FY)	1997 – 1999
Keywords	matrix metalloproteinase / inflammatory cytokines / keratocyte / superoxide dismutase
Research Abstract	We previously reported that pseudomonas elasetse, alkaline protease, exotoxinA, and LPS, all enhanced the release of both matrix metalloproteinase (MMP)-2 and MMP-9 from cultured rabbit keratocytes. In his study we indicated that inflammatory cytokines (TFN-a, IL-1b) also enhanced the release of the MMPs from cultured rabbit keratocyte. We also confirmed inhibitory effects of superoxide dismutase (SOD) on polymorphonuclear leukocyte(PMN)-induced damage of corneal tissue in guinea pigs. The inhibitory effects of SOD were indicated that corneal damage may be induced by superoxide derived from infiltrating PMN as well as MMPs from keratocyte.