1998 Fiscal Year Final Research Report Summary
Protection against oxidative stress by polyunsaturated fatty acids
| Project/Area Number |
09672257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KIKUGAWA Kiyomi School of Pharmacy, Tokyo University of Pharmacy and Life Science, Professor, 薬学部, 教授 (90120146)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Reactive oxyten species / Oxidative stress / Polyunsaturated fatty acid / Lipid peroxidation / Aging / Thiobarbituric acid test |
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| Research Abstract |
1. Use and limitation of thiobarbituric acid (TBA) test for lipid peroxidation was clarified.
2. Wistar male rats were fed a diet containing n-6 fatty acid-rich safflower oil or n-3 fatty acid-rich fish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid content in erythrocyte membranes of rats fed fish oil was significantly higher than that of rats fed safflower oil. The degree of membrane lipid peroxidation and protein aggregation of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides, TBA-reactive substances and detergent-insoluble protein aggregaates were measured. If a sufficient amount of vitamin E is supplied, n-3 fatty acid supplementation may give no undeseirable oxidative effects on rat erythrocytes in the circulation.
3. Wistar male rats were fed a diet containing safflower oil orfish oil with an equal amunt of vitamin E for 6 weeks. n-3 Fatty acid contents in lipids in brain, liver, lung, heazt, kidney, spleen, stomach ant testis were significantly higher than those of rats fed safflower oil. The degree of lipid peroxidation of the organs of ras fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroperoxides and TBA-reactive substances were measured. n-3 Fatty acid supplementation may give no undeseirable oxidative effects on rat organs.
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