1998 Fiscal Year Final Research Report Summary
Molecular analysis of neural differentiation induced by glycosyltransferase
| Project/Area Number |
09680639
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The Institute of Physical and Chemical Research (RIKEN) |
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Principal Investigator |
TSUJI Shuichi RIKEN,Molecular Glycobiology, Chief Scientist, 糖遺伝情報研究チーム, チームリーダー(研究職) (90124677)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Naoya RIKEN,Molecular Glycobiology, Scientist, 糖遺伝情報研究チーム, 研究員 (30183338)
HONG Liu 理化学研究所, 糖遺伝情報研究チーム, 研究員
TAKASHIMA Shou RIKEN,Molecular Glycobiology, Scientist, 糖遺伝情報研究チーム, 研究員 (00300880)
LIU Hong RIKEN,Molecular Glycobiology, Scientist
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| Project Period (FY) |
1997 – 1998
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| Keywords | GD3 synthase / Differential display / Neuro2a / Neural differentiation |
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| Research Abstract |
It was reported by our group that the transfection of GD3 synthase cDNA into Neuro2a cells, a neuroblastoma cell line, caused cell differntiation with neurite sprouting (J.Biol. Chem. 269, 30451-30456 (1994)). To further explore this phenomena in ditail, we applied tetracycline-regulated system to control the expression of GD3 synthase cDNA in Neuro2a cells. Under this system, Neuro2a cells also differentiated to neural cells. After that by using the differential display system, we identified 10 mRNAs that are expressed highly at the Neuro2a differentiated stage. These cDNAs were named as GDAP1-GDAP10 (ganglioside-induced differentiation-associated protein) cDNAs. It is interesting that in retinoic acid-induced neural differentiated mouse embryonic carcinoma P19 cells, GDAP mRNA expression levels were also up-regulated, ranging from three to >10 times compared with nondifferentiated P19 cells. All the GDAP genes (except that of GDAP3) were developmentally regulated. The GDAP1, 2, 6, 8, and 10 mRNAs were expressed highly in the adult mouse brain, whereas all the other GDAP mRNAs were expressed in most tissues. Our results suggested that these GDAP genes might be involved in the signal transduction pathway that is triggered through the expression of a single sialyltransferase gene to induce neural differentiation of Neuro2a cells.
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