1998 Fiscal Year Final Research Report Summary
Gene analysis of pectral finless mutant (pl) of Japanese Medaka(Oryzias latipes)
| Project/Area Number |
09680723
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nagoya University |
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Principal Investigator |
YODA Kinnya Nagoya University Bioscience Center, Assistant, 生物分子応答研究センター, 助手 (30126916)
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| Co-Investigator(Kenkyū-buntansha) |
植村 和彦 国立科学博物館, 地学研究部, 研究員 (50000138)
鈴木 三男 東北大学, 大学院・理学研究科, 教授 (80111483)
高田 克彦 九州大学, 農学部, 助手 (50264099)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Oryzias latipes / pectral finless mutant (pl) / left pectral finless mutant (lpl) / backcross / gemetic linkage map / RAPD method / 球果植物綱 / 染色体他* / 葉緑体DNA / RAPD法 / DNA塩基配列 / 核DNA / RAPD |
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| Research Abstract |
(a)backcross HNI/pl (female) x lp/pl (male): F1 offsprings (597) were obtained from 3 sets of backcross pair. Among them wildtype were 278 (46.6 %), pl=317 (53.1 %) and lpl=2 (0.3 %). We checked 500 offsprings more and found that frequency of appearance of lpl was about the same. The frequency of lpl appearance seems to be high.
(b) lpl-l (male) x lpl-2 (female): pl=90 (53 %), lpl=43 (25 %), wild type=38 (22 %), We observed early developmental stage of each embryos and found that approximately half (47) of the pl formed fin bud at early embryo stage and they disappeared after then.
(c) lpl-1 x pl/pl: The ratio of pl to lpl was not equal but most of the offsprings were pl and approximately 10 % were lpl. From these results we speculated that expression of lpl mutant might not be regulated by single gene but more than two genes take part in this event.
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