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2000 Fiscal Year Final Research Report Summary

Cell adhesion molecules and cytokines in periodontal disease

Research Project

Project/Area Number 10307054
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka Dental University

Principal Investigator

TANAKA Akio  OSAKA DENTAL UNIVERSITY, SCHOOL OF DENTISTRY, PROFESSOR, 歯学部, 教授 (10121823)

Co-Investigator(Kenkyū-buntansha) OTSUKA Kichibei  NIHON UNIVERSITY SCHOOL OF DENTISTRY, PROFESSOR, 歯学部, 教授 (50059995)
YAJIMA Toshihiko  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO, SCHOOL OF DENTISTRY, PROFESSOR, 歯学部, 教授 (10018749)
SAITO Kazuko  NIPPON DENTAL UNIVERSITY AT NIIGATA, SCHOOL OF DENTISTRY, PROFESSOR, 新潟歯学部, 教授 (30008247)
MAITA Eikichi  TOHOKU UNIVERSITY GRADUATE SCHOOL OF DENTISTRY, ASSOCIATE PROFESSOR, 大学院・歯学研究科, 助教授 (80108547)
KOGA Toshihiko  KYUSHU UNIVERSITY FACULTY OF DENTAL SCIENCE, PROFESSOR, 大学院・歯学研究院, 教授 (10037541)
Project Period (FY) 1998 – 2000
KeywordsCYTOKINE / CELL ADHESION MOLECULE / IMMUNOCOMPETENT CELL / APOPTOSIS / EXTRACELLULAR MATRIX / FIBROBLAST / EPITHELIAL CELL / PROSTAGLANDIN
Research Abstract

We studied the role of cell adhesion molecules and cytokines in periodontal diseases, and obtained the following results. The primary difference in the characterization of both cells and matrix was in the adhesion between fibroblasts and extracellular matrix. Both CD43 and a glycoprotein of about 45-kDa played roles in the adhesion sequence between fibroblasts and immunocompetent cells. Fibroblasts derived from hyperplastic gingiva produced abundant quantities of both TGF-β and IL-6 when stimulated by LPS.In rat experimental periodontitis, leukocyte chemotactic factor was produced not only by inflammatory cells but also by junctional epithelial cells. There was a direct correlation between the amount of hepatocyte growth factor in gingival crevicular fluid and other parameters of periodontal disease such as probing depth, gingival index and amount of IL-1β. Among the several PGE2 receptors expressed on fibroblasts, EP2 and EP4 receptors were identified as those involved with the inhibi … More tion of ICAM-1 expression. Stimulation by HSP60 of mononuclear cells from peripheral blood of patients with advanced periodontitis produced significantly increased expression of IFN-γ at both the mRNA and protein levels. The ratio of IFN-γ derived from mononuclear cells within periodontally involved gingival tissues to that of mononuclear cells from peripheral blood of the same patients was lower, whereas the respective ratio for IL -4 was higher. There was interruption of apoptosis within lymp hocytes from gingiva involved with periodontitis due to diminished signal transfer as well as molecular apoptosis inhibition. Peritoneal re-infection of rats immunized to A.actinomycetemcomitans resulted in increased production of both TNF-α and IL-6, thus leading to markedly increased production of IFN-γ. Five serotypes, a through e, of A.actinomycetemcomi tans were genetically identified. PCR techniques and base sequence analysis have led to methods of serotype identification that are very useful in understanding the pathogenesis and diagnosis of periodontal disease. Less

  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] Yamanegi K et al.: "Application of mechanically-dispersed human gingival tissues to flow cytometry"Dent Jpn. 36巻. 113-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中昭男: "歯周病と生体防御作用-発症と治療における生体防御因子の関与-:歯周病罹患歯肉にみられる細胞の機能"日本歯科医学会誌. 19巻. 104-107 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 長曾善彦 他: "A.actinomycetemcomitansに対するマウスの一次免疫応答におけるα/βT細胞の役割とサイトカイン産生"歯学. 87巻. 247-260 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato C et al.: "Nitric oxide production and iNOS nRNA expression in mice induced by repeated stimulation with live Fusobacterium nucleatum"Microbiol Immunol. 45巻. 69-78 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor is a predominant chemoattracttant for gingival epithelial cells produced by radicular cyst-derived fibrobolast-like cells"J Oral Sci. 42巻. 101-106 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima M et al.: "Profiles of cytokine expression in radicular cyst-lining epithelium examined by RT-PCR"J Oral Sci. 42巻. 239-246 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor secreted by periodontal ligament and gingival fibroblasts is a major chemoattractant for gingival epithelial cells"J Periodont Res. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor (HGF) in periodontal disease : detection of HGF in gingival crevicular fluid"J Periodont Res. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida Y et al.: "A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose"J Biol Chem. 274巻. 16933-16939 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito T et al.: "Association between periodontitis and exercise capacity"Periodont Insights. 6巻. 9-12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida Y et al.: "Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans"Biochim Biophys Acta. 1489巻. 457-461 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida A et al.: "A novel dnaK operon from Porphyromonas gingivalis"FEBS Letters. 446巻. 287-291 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano Y et al.: "Thymidine diphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans"J Biol Chem. 275巻. 6806-6812 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano Y et al.: "A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans"Biochim Biophys Acta. 1493巻. 259-263 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsushita K et al.: "The role of vascular endothelial growth factor in human dental pulp cells : induction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway"J Dent Res. 79巻. 1596-1603 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki N et al.: "Genetic analysis of the gene cluster for the synthesis of serotype a-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans."Biochim Biophys Acta. 1517巻. 135-138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimura M et al.: "Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis"Infect Immun. 68巻. 6912-6916 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida A et al.: "Immunodominant region of Actinobacillus actinomycetemcomitans 40-kilodalton heat shock protein in patients with rheumatoid arthritis"J Dent Res. 80巻. 346-350 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakuta T et al.: "Enhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1"J Med Microbiol. 50巻. 233-237 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tabeta K et al.: "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts"Infect Immun. 68巻. 3731-3735 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tabeta K et al.: "Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients"Clin Exp Immunol. 120巻. 285-293 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki K et al.: "Selective expansion of T cells in gingival lesion of patients with chronic inflammatory periodontal disease"Clin Exp Immunol. 120巻. 154-161 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村上伸也 他: "ヒアルロン酸の生物学的活性を考える"炎症. 19巻. 307-318 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi K et al.: "Cell synthesis, proliferation and apoptosis in human dental periapical lesions analysed by in situ hybridization and immunohistochemistry"Oral Dis. 5巻. 313-320 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawa T et al.: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesions by exogenous Fas ligand"Infect Immun. 67巻. 1450-1454 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Guo S et al.: "Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis"J Clin Periodontol. 27巻. 769-777 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi K et al.: "Heterogeneity of host immunological risk factors in patients with aggressive periodontitis"J Periodontol. 72巻. 425-437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaki K et al.: "Prostaglandin E_2 and I_2 regulate intercellular adhesion molecule-1 expression in interleukin-1β-stimulated human gingival fibroblasts"J Periodont Res. 34巻. 97-104 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin E2 downregulates interferon-γ-induced intercellular adhesion molecule-1 expression via EP_2 receptors in human gingival fibroblasts"Inflammation. 23巻. 481-493 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi K et al.: "Involvement of cyclooxygenase-2 in interleukin-1 α-induced prostaglandin production by human periodontal ligament cells"J Periodontol. 70巻. 902-908 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin F2alpha upregulates intercellular adhesion molecule-1 expression in human gingival fibroblasts"J Periodont Res. 34巻. 277-281 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin E_2 receptors of the EP_2 and EP_4 subtypes downregulate tumor necrosis factor α-induced intercellular adhesion molecule-1 expression in human gingival fibroblasts"J Periodont Res. 34巻. 478-485 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamanegi K et al.: "Application of mechanically-dispersed human gingival tissues to flow cytometry"Dent Jpn. 36. 113-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka A: "Host defense in periodontal disease : the role of host-defense factors in the progression and treatment of disreas : cell functioin in the gingiva during periodontal disease (Japanese)"Nippon Shika Igakkai Shi (J Jpn Assoc Dent Sci). 19. 104-107 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagaso Y et al.: "Role of α/β T cell and cytokine production in primary response of mouse against A.actinomycetem-comitans (Japanese)"Shigaku (Odontology). 87 (2). 247-260 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato C et al.: "Nitric oxide production and iNOS nRNA expression in mice induced by repeated stimulation with live Fusobacterium nucleatum"Microbiol Immunol. 45 (1). 69-78 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor is a predominant chemoattracttant for gingival epithelial cells produced by radicular cyst-derived fibrobolast-like cells"J Oral Sci. 42 (2). 101-106 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima M et al.: "Profiles of cytokine expression in radicular cyst-lining epithelium examined by RT-PCR"J Oral Sci. 42 (4). 239-246 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor secreted by periodontal ligament and gingival fibroblasts is a major chemoattractant for gingival epithelial cells"J Periodont Res. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima M et al.: "Hepatocyte growth factor (HGF) in periodontal disease : detection of HGF in gingival crevicular fluid"J Periodont Res. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida Y et al.: "A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose"J Biol Chem. 274 (24). 16933-16939 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito T et al.: "Association between periodontitis and exercise capacity"Periodont Insights. 6 (2). 9-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida Y et al.: "Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans"Biochim Biophys Acta. 1489 (2/3). 457-461 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida A et al.: "A novel dnaK operon from Porphyromonas gingivalis"FEBS Letters. 446 (2/3). 287-291 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano Y et al.: "Thymidinediphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans"J Biol Chem. 275 (10). 6806-6812 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano Y et al.: "A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans"Biochim Biophys Acta. 1493 (1/2). 259-263 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsushita K et al.: "The role of vascular endothelial growth factor in human dental pulp cells : induction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway"J Dent Res. 79 (8). 1596-1603 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki N et al.: "Genetic analysis of the gene cluster for the synthesis of serotype a-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans"Biochim Biophys Acta. 1517 (1). 135-138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimura M et al.: "Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis"Infect Immun. 68 (12). 6912-6916 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida A et al.: "Immunodominant region of Actinobacillus actinomycetemcomitans 40-kilodalton heat shock protein in patients with rheumatoid arthritis"J Dent Res. 80 (1). 346-350 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakuta T et al.: "Enhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1"J Med Microbiol. 50 (3). 233-237 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tabeta K et al.: "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts"Infect Immun. 68 (6). 3731-3735 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tabeta K et al.: "Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients"Clin Exp Immunol. 120 (2). 285-293 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki K et al.: "Selective expansion of T cells in gingival lesion of patients with chronic inflammatory periodontal disease"Clin Exp Immunol. 120 (1). 154-161 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami S et al.: "Biological antivities of hyaluronan (Japanese)"Ensho (Jpn J Inflamm). 19 (6). 307-318 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi K et al.: "Cell synthesis, proliferation and apoptosis in human dental periapical lesions analysed by in situ hybridization and immunohistochemistry"Oral Dis. 5 (4). 313-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawa T et al.: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesions by exogenous Fas ligand"Infect Immun. 67 (3). 1450-1454 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Guo S et al.: "Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis"J Clin Periodontol. 27 (10). 769-777 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi K et al.: "Heterogeneity of host immunological risk factors in patients with aggressive periodontitis"J Periodontol. 72 (4). 425-437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaki K et al.: "Prostaglandin E_2 and I_2 regulate intercellular adhesion molecule-1 expression in interleukin-1β-stimulated human gingival fibroblasts"J Periodont Res. 34 (2). 97-104 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin E2 downregulates interferon-γ-induced intercellular adhesion molecule-1 expression via EP_2 receptors in human gingival fibroblasts"Inflammation. 23 (5). 481-493 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi K et al.: "Involvement of cyclooxygenase-2 in interleukin-1α-induced prostaglandin production by human periodontal ligament cells"J Periodontol. 70 (8). 902-908 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin F2alpha upregulates intercellular adhesion molecule-1 expression in human gingival fibroblasts"J Periodont Res.. 34 (5). 277-281 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi K et al.: "Prostaglandin E_2 receptors of the EP_2 and EP_4 subtypes downregulate tumor necrosis factor α-induced intercellular adhesion molecule-1 expression in human gingival fibroblasts"J Periodont Res. 34 (8). 478-485 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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