2000 Fiscal Year Final Research Report Summary
Study on B cell differentiation signaling by using a novel system
| Project/Area Number |
10470091
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Tokyo Medical and Dental University (2000)
Tokyo Metropolitan Organization for Medical Research (1998-1999) |
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Principal Investigator |
KARASUYAMA Hajime Tokyo Medical and Dental University, Dept. of Immune Regulation, Professor, 大学院・医歯学総合研究科, 教授 (60195013)
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| Co-Investigator(Kenkyū-buntansha) |
MORIO Tomohiro Tokyo Medical and Dental University, Dept. of Developmental immunology, Assistant, 大学院・医歯学総合研究科, 助手 (30239628)
SATO Shingo Tokyo Medical and Dental University, Dept. of Immune Regulation, Assistant, 大学院・医歯学総合研究科, 助手 (10143562)
MAKI Kazushige Tokyo Medical and Dental University, Dept. of Immune Regulation, Assistant, 大学院・医歯学総合研究科, 助手 (10311424)
SORIMACHI Noriko Tokyo Medical and Dental University, Dept. of Immune Regulation, Lecturer, 大学院・医歯学総合研究科, 講師 (30217468)
SHIGEAKI Nonoyama Tokyo Medical and Dental University, Dept. of Developmental immunology, Lecturer, 大学院・医歯学総合研究科, 講師 (40280961)
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| Project Period (FY) |
1998 – 2000
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| Keywords | per B cell receptor (preBCR) / B cell differentiation / pro B cell / pre B cell / Igβ / signal transduction / gene cloning / raft |
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| Research Abstract |
Pre B cell receptor (preBCR) is expressed transiently at the early stage of B cell development and plays an important role in B cell differentiation. It remains to be determined what kind of signals are delivered from preBCR to induce B cell differentiation. In this study, we examined the preBCR signal transduction pathway by using a novel system which we have originally developed. We purified and sequenced a 36 kD protein (pp36) that was tyrosine-phosphorylated by preBCR signaling. The cloning of a corresponding cDNA revealed that pp36 is a novel protein with lipid modification at the N-terminal region. Since this lipid modification is know to be essential for proteins to anchor into lipid raft, pp36 appears to play an important role in signal transduction.
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[Publications] Endo, J., Toyama-Sorimachi, N., Taya, C., Kuramochi-Miyagawa, S., Nagata, K., Kuida, K., Takashi, T., Yonekawa, H., Yoshizawa, Y., Miyasaka, N.and Karasuyama, H.: "Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-l clustering and cell adhesion of activated lymphocytes."FEBS lett. 468. 234-238 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Donohoe, M.E., Beck-Engeser, G.B., Lonberg, N.Karasuyama, H., Riley, R.L., Jack, H-M., and Blomberg, B.B.: "Transgenic human λ5 rescues the murine λ5 nullizygous phenotype."J.Immunol.. 164. 5269-5276 (2000)
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[Publications] Nomura, K., Kanegane, H., Karasuyama, H., Tsukada, S., Agematsu, K., Murakami, G., Sakazume, S., Sako, M., Tanaka, R., Kuniya, Y., Komeno, T., Ishihara, S., Hayashi, K., Kishimoto, T.and Miyawaki, T.: "Genetic defect in human X-linked agammaglobulinemia impedes a maturational evolution of pro-B cells into a later stage of pre-B cells in the B-cell differentiation pathway."Blood. 96. 610-617 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Nagaoka, H., Takahashi, Y., Hayashi, R., Nakamura, T., Ishii, K., Matsuda, J., Ogura, A., Shirakata, Y., Karasuyama, H., Sudo, T., Nishikawa, S., Tsubata, T., Mizuochi, T., Asano, T., Sakano, H.and Takemori, T.: "Ras mediates effector pathways responsible for pre-B cell survival, which is essential for the developmental progression to the late pre-B cell stage."J.Exp.Med.. 192. 171-182 (2000)
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「研究成果報告書概要(欧文)」より
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