2000 Fiscal Year Final Research Report Summary
Function and regulation of intermediate filament organization during cell prolifenation and cell signalings.
| Project/Area Number |
10480205
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
INAGAKI Masaki Division of Biochemistry Chief, Aichi Cancer Center, 発がん制御研究部, 部長 (30183007)
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| Co-Investigator(Kenkyū-buntansha) |
INADA Hiroyasu Division of Biochemistry Section Researcher, Aichi Cancer Center, 発がん制御研究部, 研究員 (90283522)
NAGATA Koichi Division of Biochemistry Section Head, Aichi Cancer Center, 発がん制御研究部, 室長 (50252143)
NAGATA Koichi Division of Biochemistry Section Head, Aichi Cancer Center (50252143)
NAGATA Koichi Division of Biochemistry Section Head, Aichi Cancer Center (50252143)
INADA Hiroyasu Division of Biochemistry Section Researcher, Aichi Cancer Center (90283522)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Phosphorylation / Cleavage furrow / Phosphopeptide antibodies / Rho / Cytokinesis / Intermediate filament (IF) / Rho-associated kinase (Rho-kinase) |
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| Research Abstract |
Intermediate filaments (IFs), which form the structural framework of cytoskeleton, have been found to be dramatically reorganized during mitosis. Some protein kinases activated in mitosis are thought to control spatial and temporal IF reorganization through phosphorylation of IF proteins. Rho-associated kinase (Rho-kinase), one of. the putative targets of the small GTPase Rho, does phosphorylate IF proteins, specifically at the cleavage furrow during cytokinesis. This cleavage furrow-specific phosphorylation plays an important role in the local IF breakdown and efficient separation of IF networks, Recent studies on Rho signaling pathways have allowed new models about the molecular mechanism of rearrangements of cytoskeletons including IFs during cytokinesis.
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[Publications] Yasui, Y., Amano, M., Nagata, K., Inagaki, N., Nakamura, H., Saya, H., Kaibuchi, K., and Inagaki, M.: "Roles of Rho-associated kinase in cytokinesis ; Mutations in Rho-associated kinase phosphorylation sites impair cytokinetic segregation of glial filaments."J.Cell Biol.. 143. 1249-1258 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kosako, H., Goto, H., Yanagida, M., Matsuzawa, K., Fujita, M., Tomono, Y., Okigaki, T., Odai, H., Kaibuchi, K., and Inagaki, M.: "Specific accumulation of Rho-associated kinase at the cleavage furrow during cytokinesis ; Cleavage furrow-specific phosphorylation of intermediate filaments."Oncogene. 18. 2783-2788 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Goto, H., Tomono, Y., Ajiro, K., Kosako, H., Fujita, M., Sakurai, M, Okawa, K., Iwamatsu, A., Okigaki, T., Takahashi, T., and Inagaki, M.: "Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation."J.Biol. Chem. 274. 25543-25549 (1999)
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[Publications] Inagaki, N., Nishizawa, M., Minakata, Y., Yamamoto, H., Takeuchi, Y., Miyamoto, E., Kaibuchi, K., and Inagaki, M.: "Activation of Ca^<2+>/calmodulin-dependent protein kinase llwithin post-synaptic dendritic spines of cultured hippocampal neurons."J.Biol. Chem. 275. 27165-27171 (2000)
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[Publications] Izawa, I., Nishizawa, M., Ohtakara, K., Ohtsuka, K., Inada, H., and Inaqaki, M.: "Identification of Mrj, a DnaJ/Heat shock protein 40family protein, as akeratin 8/18 filament-regulatory protein."J.Biol. Chem. 275. 34521-34527 (2000)
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[Publications] Togashi, H., Nagata, K., Takagishi, M., Saitoh, M., and Inagaki, M.: "Functions of a Rho-specific guanine nucleotide-exchange factor, in neurite retraction : Possible involvement ofproline-rich motif of KIAA0380 in the localization."J.Biol. Chem.. 275. 29570-29578 (2000)
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「研究成果報告書概要(欧文)」より
