2000 Fiscal Year Final Research Report Summary
Studies on Neuronal mRNA Splicing
| Project/Area Number |
10480212
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
YASUHARA Osamu Molecular Neuroscience Research Center, Shiga University of Medical Science Associate Professor, 分子神経科学研究センター, 助教授 (80239772)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Molecular Neuroscience Research Center, Shiga University of Medical Science Professor, 分子神経科学研究センター, 教授 (40079736)
TOOYAMA Ikuo Molecular Neuroscience Research Center, Shiga University of Medical Science Professor, 分子神経科学研究センター, 教授 (20207533)
AIMI Yoshinari Molecular Neuroscience Research Center, Shiga University of Medical Science Reserch Associate, 分子神経科学研究センター, 助手 (20231756)
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| Project Period (FY) |
1998 – 2000
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| Keywords | alternative splicing / choline acetyltransferase / cholinergic neurons / retinal ganglion cells / antisense oligonucleotide / immunohistochemistry / parasympathetic nervous system / RT-PCR |
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| Research Abstract |
Acetylcholine is a neurotransmitter in the central and peripheral nervous systems. Cholinergic neurons have been demonstrated by immunohistochemistry using antibodies to choline acetyltransferase (ChAT). Although most of such antibodies can clearly stain central cholinergic neurrons, they generally fail to demonstrate peripheral cholinergic neurons. We have recently found that peripheral cholinergic cells preferentially express an alternative splice variant of ChAT mRNA (pChAT mRNA). In the present research project, we have reevaluated both the central and peripheral cholinergic nervous system using the antibody to pChAT.The pChAT antibody stained peripheral parasympathetic neurons. In addition, certain populations of neurons in the central and peripheral nervous systems, which have never been regarded as a part of the cholinergic system, have been recognized to be pChAT-positive. They include primary sensory neurons in the dorsal root ganglia, retinal ganglion cells, and neurons in the tubelomammillary hypothalamic nucleus. We have also confirmed the expression of pChAT mRNA in such areas. The discovery of pChAT should facilitate investigations on the periphearl, as well as central, cholinergic systems.
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[Publications] Aisaka, A., Aimi, Y., Yasuhara, O., Tooyama, I., Kimura, H., Shimada, M.: "Two modes of corticospinal reinnervation occur close to spinal targets following unilateral lesion of the motor cortex in neonatal hamsters."Neuroscience. 90. 53-67 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Tooyama, I., Nishimura, T., Nakagawa, E, Morita, H., Uemura, S., Aimi, Y., Yasuhara, O., Kimura, H.: "Microglial reaction and neuronal death in the hippocampus of rat models of epilepsy."Neuropathology. 19. 203-208 (1999)
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[Publications] Nakanishi, Y., Tooyama, I., Yasuhara, O., Aimi, Y., Kitajima, K., Kimura, H.: "Immunohistochemical localization of choline acetyltransferase of a peripheral type in the rat larynx."J.Chem.Neuroanat. 17. 21-32 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakagawa, E., Aimi, Y., Yasuhara, O., Tooyama, I., Shimada, M., McGeer, P.L., Kimura, H.: "Enhancement of progenitor cell division in the dentate gyrus triggered by initial limbic seizures in rat models of epilepsy."Epilepsia. 41. 10-18 (2000)
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「研究成果報告書概要(欧文)」より
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