1999 Fiscal Year Annual Research Report

新興・再興感染症として重要な包虫症・襄虫症に関する遺伝子・免疫迅速診断法の研究

Research Project

Project/Area Number	10557029
Research Institution	ASAHIKAWA MEDICAL COLLEGE
Principal Investigator	伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)
Co-Investigator(Kenkyū-buntansha)	中尾稔旭川医科大学, 医学部・寄生虫学, 助手 (70155670) 岡本宗裕鳥取大学, 農学部獣医学科・実験動物機能学, 助教授 (70177096) 堀井俊宏大阪大学, 微生物病研究所・分子原虫学, 教授 (80142305) 中谷和宏旭川医科大学, 医学部・動物実験施設, 教務職員 (70109388) 迫康仁旭川医科大学, 医学部・寄生虫学, 助手 (40312459)
Keywords	新興・再興感染症 / エキノコックス症 / 多包虫症 / 単包虫症 / 有鉤襄虫症 / 鑑別血清診断法 / ミトコンドリアDNA / 遺伝子診断法
Research Abstract	新興・再興感染症として世界的規模で流行、汚染地域拡大が年々深刻化してきている寄生虫病にテニア科条虫の幼虫が人体ならびに家畜動物に寄生することによって惹起される包虫症(単包虫症、多包虫症)と襄虫症とがある。いずれも難治性寄生虫疾患で、死の転帰を取ることが非常に多く、早期鑑別診断法の確立は国際的ニーズが高い緊急を要する研究課題である。本研究ではこれらの鑑別を要する寄生虫病について旭川医科大学寄生虫学講座におけるこれまでの実績に基づく国際共同研究を展開することによって(1)世界的流行の現状把握につとめ、(2)多包虫症、単包虫症、有鉤襄虫症それぞれの鑑別診断法の原法をまず確立した。その上で(3)鑑別診断用抗原精製法に検討を加え、(4)遺伝子組換え抗原作製を試み、(5)国際的に最も信頼性が高いと評価される多包虫症、単包虫症、有鉤襄虫症それぞれについて血清学的鑑別診断法を確立している。これらの成績に基づき(6)世界各地における流行地域での疫学調査研究への参加を要請されている。さらに(7)病巣から摘出されるエキノコックス標本あるいは襄虫標本、さらには人体から排出されるテニア科条虫受胎片節、虫卵などを用いた遺伝子鑑別診断法の確立の基礎となる多包虫、有鉤襄虫ミトコンドリアDNA全塩基配列の解読に世界で最初に成功し、(8)条虫が利用しているユニークな遺伝子暗号の読み始め(ATGとGTG)と読み終えに利用しているコドン(TAGとTAA)を発見したことが最大の基礎研究成果である。(9)分子疫学研究あるいは(10)寄生虫と宿主の共進化と世界拡散についての遺伝子レベルの研究展開が今後の基礎研究課題である。昨年9月にアルゼンチンで開催されただい19回国際エキノコックス症会議に本研究代表者、伊藤亮が「多包虫症単包虫症、有鉤襄虫症についての血清学的鑑別診断法」と題する招聘講演を要請され、この発表論文が会議における最優秀論文賞に選ばれている。

Research Products

(23 results)

All Other

All Publications (23 results)

[Publications] Ito A.,Nakao M.,Sako Y.,Nakaya K.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health. (in press). (2000)
[Publications] Wandra T.,Subahar R.,Simaniuntak GM.,Margono SS.,Suroso T.,Okamoto M.,Nakao M.,Sako Y.,Nakaya K.,Schantz PM.,Ito A.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia, 1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 1-5 (2000)
[Publications] Ito A.,Plancarte A.,Nakao M.,Nakaya K.,Ikejima T.,Piao ZX.,Kanazawa T.,Margono SS.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)
[Publications] Qiu JM.,Liu FJ.,Wang H.,Ito A.,Schantz PM.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)
[Publications] Ito A.: "Differential serodiagnosis for larval cestode infections : cystic echinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)
[Publications] Kimura M.,Nakamura T.,Iwamoto A.,Nishimura Y.,Egawa T.,Ito A.: "Cystic echinococcosis in a Jordanian patient : albendazole in a short-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)
[Publications] Wang IC.,Ma YX.,Guo JX.,Chung WC.,Lu SC.,Ito A.,Fan PC.: "Oncospheres of Taenia solium and T. saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)
[Publications] Willingham III AL.,Johansen MV.,Bogh HO.,Ito A.,Andreassen J.,Lindberg R.,Christensen NO.,Nansen P.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)
[Publications] Ito A.,Ito M.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)
[Publications] Ohsaki Y.,Matsumoto A.,Miyamoto K.,Kondoh N.,Araki K.,Ito A.,Kikuchi K.: "Neurocysticercosis without detectable specific antibody"Internal Medicine. 38. 67-70 (1999)
[Publications] Ito A.,Nakao M.,Ito Y.,Yuzawa I.,Morishima H.,Kawano N.,Fujii K.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)
[Publications] Ito A.,Ma L.,Schantz PM.,Gottstein B.,Liu YH.,Chai JJ.,Abder-Hafez SK.,Altintas N.,Joshi DD.,Lightowlers MW.,Pawlowski ZS.: "Differential serodiagnoses for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E.. multilocularis protoscolex (Em18)"American Journal of Tropical Medicine and Hygiene. 60. 188-192 (1999)
[Publications] Ito A.,Plancarte A.,Ma L.,Kong Y.,Flisser A.,Cho YS.,Liu YH.,Kamhawi S.,Lightowlers MW.,Schantz PM.: "Novel antigens for neurocysticerosis : simple method for preparation and evaluation for serodiagnosis"American Journal of Tropical Medicine and Hygiene. 59. 291-294 (1998)
[Publications] Niwa A.,Asano K.,Ito A.: "Eosinophil chemotactic factors from cysticercoids of Hymenolepis nana"Journal of Helminthology. 72. 273-275 (1998)
[Publications] Yang HJ.,Chung JY.,Yun DH.,Kong Y.,Ito A.,Ma L.,Liu YH.,Lee SC.,Kang SY.,Cho SY.: "Immunoblot anyalysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes"Parasite Immunology. 20. 483-488 (1998)
[Publications] Kong Y.,Ito A.,Yang HJ.,Chung YB.,Kasuya S.,Kobayashi M.,Liu YH.,Cho SY.: "Immunoglobulin G(IgG) subclass and IgE responses in human paragonimiases caused by three different species"Chinical and Diagnostic Laboratory Immunology. 5. 474-478 (1998)
[Publications] Ito A.,Ma L.,Paul M.,Stefaniak J.,Pawlowski ZS.: "Evaluation of Em18-, Em16-, Antigen B-Western blots, Em2^<plus>-ELISA and four other tests for differential serodiagnosis of alveolar and cystic echinococcosis patients in Poland"Parasitology International. 47. 95-99 (1998)
[Publications] Ito A.,Okamoto M.,Ishiguro T.,Ma L.,Suzuki H.,Yasui A.,Ahigeta H.,Matsuura T.,Hosokawa T.,Chai JJ.: "An imported case of cystic echinococcosis in Japan diagnosed by imaging and serology with confirmation of Echinococcus granulosus-specific DNA sequences"American Journal of Tropical Medicine and Hyginene. 58. 790-792 (1998)
[Publications] Ito A.,Sakakibara Y.,Ma L.,Asano K.,Takiguchi M.,Yasuda J.,Hashimoto A.: "Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis"Parasite Immunology. 20. 105-110 (1998)
[Publications] Nakaya K.,Oomori Y.,Kutsumi H.,Nakao M.: "Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole"Journal of Helminthology. 72. 349-354 (1998)
[Publications] Sato Y.,Nakao M.,Nakaya K.,Ito A.: "Experimental infection of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis"Journal of Helminthology. 72. 59-64 (1998)
[Publications] 伊藤亮: "日本における寄生虫学の研究第7巻6章"目黒寄生虫館. 684 (1999)
[Publications] 辻守康、近藤力王至、伊藤亮: "日本における寄生虫学の研究第6巻5章"目黒寄生虫館. 672 (1999)

1999 Fiscal Year Annual Research Report

新興・再興感染症として重要な包虫症・襄虫症に関する遺伝子・免疫迅速診断法の研究

Principal Investigator

伊藤 亮 旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

Research Products

[Publications] Ito A.,Nakao M.,Sako Y.,Nakaya K.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health. (in press). (2000)

[Publications] Ito A.,Plancarte A.,Nakao M.,Nakaya K.,Ikejima T.,Piao ZX.,Kanazawa T.,Margono SS.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)

[Publications] Qiu JM.,Liu FJ.,Wang H.,Ito A.,Schantz PM.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)

[Publications] Ito A.: "Differential serodiagnosis for larval cestode infections : cystic echinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)

[Publications] Kimura M.,Nakamura T.,Iwamoto A.,Nishimura Y.,Egawa T.,Ito A.: "Cystic echinococcosis in a Jordanian patient : albendazole in a short-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)

[Publications] Wang IC.,Ma YX.,Guo JX.,Chung WC.,Lu SC.,Ito A.,Fan PC.: "Oncospheres of Taenia solium and T. saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)

[Publications] Willingham III AL.,Johansen MV.,Bogh HO.,Ito A.,Andreassen J.,Lindberg R.,Christensen NO.,Nansen P.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)

[Publications] Ito A.,Ito M.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)

[Publications] Ohsaki Y.,Matsumoto A.,Miyamoto K.,Kondoh N.,Araki K.,Ito A.,Kikuchi K.: "Neurocysticercosis without detectable specific antibody"Internal Medicine. 38. 67-70 (1999)

[Publications] Ito A.,Nakao M.,Ito Y.,Yuzawa I.,Morishima H.,Kawano N.,Fujii K.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)

[Publications] Ito A.,Plancarte A.,Ma L.,Kong Y.,Flisser A.,Cho YS.,Liu YH.,Kamhawi S.,Lightowlers MW.,Schantz PM.: "Novel antigens for neurocysticerosis : simple method for preparation and evaluation for serodiagnosis"American Journal of Tropical Medicine and Hygiene. 59. 291-294 (1998)

[Publications] Niwa A.,Asano K.,Ito A.: "Eosinophil chemotactic factors from cysticercoids of Hymenolepis nana"Journal of Helminthology. 72. 273-275 (1998)

[Publications] Yang HJ.,Chung JY.,Yun DH.,Kong Y.,Ito A.,Ma L.,Liu YH.,Lee SC.,Kang SY.,Cho SY.: "Immunoblot anyalysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes"Parasite Immunology. 20. 483-488 (1998)

[Publications] Kong Y.,Ito A.,Yang HJ.,Chung YB.,Kasuya S.,Kobayashi M.,Liu YH.,Cho SY.: "Immunoglobulin G(IgG) subclass and IgE responses in human paragonimiases caused by three different species"Chinical and Diagnostic Laboratory Immunology. 5. 474-478 (1998)

[Publications] Ito A.,Ma L.,Paul M.,Stefaniak J.,Pawlowski ZS.: "Evaluation of Em18-, Em16-, Antigen B-Western blots, Em2^<plus>-ELISA and four other tests for differential serodiagnosis of alveolar and cystic echinococcosis patients in Poland"Parasitology International. 47. 95-99 (1998)

[Publications] Ito A.,Sakakibara Y.,Ma L.,Asano K.,Takiguchi M.,Yasuda J.,Hashimoto A.: "Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis"Parasite Immunology. 20. 105-110 (1998)

[Publications] Nakaya K.,Oomori Y.,Kutsumi H.,Nakao M.: "Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole"Journal of Helminthology. 72. 349-354 (1998)

[Publications] Sato Y.,Nakao M.,Nakaya K.,Ito A.: "Experimental infection of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis"Journal of Helminthology. 72. 59-64 (1998)

[Publications] 伊藤 亮: "日本における寄生虫学の研究 第7巻6章"目黒寄生虫館. 684 (1999)

[Publications] 辻守康、近藤力王至、伊藤 亮: "日本における寄生虫学の研究 第6巻5章"目黒寄生虫館. 672 (1999)

伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

[Publications] 伊藤亮: "日本における寄生虫学の研究第7巻6章"目黒寄生虫館. 684 (1999)

[Publications] 辻守康、近藤力王至、伊藤亮: "日本における寄生虫学の研究第6巻5章"目黒寄生虫館. 672 (1999)