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1999 Fiscal Year Final Research Report Summary

Extraction of Lipopolysaccaride Using Thermoresponsive Polymer

Research Project

Project/Area Number 10640596
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 分離・精製・検出法
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

MATSUBARA Chiyo  Tokyo University of Pharmacy and Life Science, School of Life Science, Professor, 生命科学部, 教授 (10057309)

Co-Investigator(Kenkyū-buntansha) SAKURAI Teruaki  Tokyo University of Pharmacy and Life Science, School of Life Science, Assistant, 生命科学部, 助手 (30266902)
SAITOH Tohru  Tokyo University of Pharmacy and Life Science, School of Life Science, Assistant Professor, 生命科学部, 講師 (40186945)
Project Period (FY) 1998 – 1999
Keywordspoly(N-isopropylacrylamide / Lipopolysaccharide(LPS) / concentration / hydrophobic / thermoresponsive / organic compound / extraction / phase separation
Research Abstract

A water-soluble polymer, poly(N-isopropylacrylamide), PNIPAAm, become waterinsoluble above a lower critical solution temperature(ca.32℃)and forms gum-like aggregates(polymer phase) having very small volume fraction.
Lipopolysaccharide(LPS) was successfully extracted by polymer-mediated extraction based on the thermoresponsive precipitation of water-soluble polymer PNIPAAm in aqueous solutions. LPS having hydrophobic lipid moiety and hydrophilic polysaccharides was less extractive than lipids but well concentrated to small volume of aggregated polymer precipitates (polymer phase). In contrast, hydrophilic compounds including saccharides, urea, creatine, serum proteins and inorganic ions hardly incorporated into the polymer phase. Corrected LPS could be recovered to KURIMOVER(chitosan beads) column by passing though the aqueous PNIPAAm solution of LPS.
The application of the polymer-mediated extraction will greatly extend the potential of this method in the pre-concentration of traces of LPS.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 齋藤徹: "温度感応性高分子を用いる水溶液中の化合物の濃縮(均一固相抽出法)"JUSCO Report. 39(2). 1-5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松原チヨ: "界面活性剤、高分子電解質を用いる溶媒抽出"ぶんせき. 1999(1). 60-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohru Saitoh: "Concentration of Hydrophobic Organic Compounds by Polymer-mediated Extraction"Analytical Chemistry. 1999. 4506-4512 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohru Saitoh: "Concentration of Compounds in Water Using a Thermally Reversible Polymer."JUSCO Report. 39(2). 1-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiyo,Matsubara: "Solvent Extraction with Surfactants and Polyelectrolytes."BUNSEKI. 1999(1). 60-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tohru Saitoh: "Concentration of Hydrophobic Organic Compounds by Polymer-mediated Extraction."Analytical Chemistry. 1999(71). 4506-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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