1999 Fiscal Year Final Research Report Summary
Proteoglycans distributed intra-and extra-cellular compartments of the central nervous system
| Project/Area Number |
10670031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Juntendo University |
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Principal Investigator |
NISHIZUKA Masako Juntendo University, School of Medicine, Assistant Professor, 医学部, 講師 (20133332)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Proteoglycan / Glia / Cerebral cortex / GABA |
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| Research Abstract |
Intra-and extra-cellular distribution of neural proteoglycans, neurocan and phosphacan, was examined using anti-neurocan/phosphacan-antibodies in the cerebral cortex of rats. Neurocan and a fragment of neurocan that consisted of a carboxyl-terminal half of neurocan was distributed extracellularly in immature rat brain and in adults, respectively. By contrast, a fragment consisting of an amino-terminal half of neurocan was distributed in the cytoplasm of the glial processes in the cortex of adults. Phosphacan was seen extracellularly in the brain of rats, but it was also seen in the cytoplasm of the glial processes in the cerebral cortex of adults.
Neurocan-or phosphacan-containing glial processes enwrapped the cell bodies of GABAergic neurons containing parvalbumin. Such glial structures corresponded to the glial peri-neuronal nets. In a number of the glial peri-neuronal nets of the cortex, phosphacan and the amino-terminal half of neurocan were co-localized in the cytoplasm. The glial peri-neuronal nets and the proteoglycans in their cytoplasm may be involved in the stabilization of microenvironment around pre-and post-synapses, in particular, of the burst-like firing characterized GABA ergic non-pyramidal interneurons containing parvalbumin in the cerebral cortex.
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