2001 Fiscal Year Final Research Report Summary

Mechanism of Megalraryocyhic differentiction-localigation of throwbpoietin receptor and ultrastructure of Megakaryocyte

Research Project

Project/Area Number	10670195
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Experimental pathology
Research Institution	The University of Tokyo
Principal Investigator	TANGE Tsuyoshi University of Tokyo, Faculty of Medicin, Associate Professor, 医学部附属病院, 助教授 (10107667)
Project Period (FY)	1998 – 2001
Keywords	Megakaryocyte / Differentiation / Thrombopoietin / C-MPl / gold-colloid / Eectron microscope
Research Abstract	In our laboratory, immuno-electron microscopyc was applied to demonstrate the location of c-MPL (thrombopoietin receptor) in human megakaryocytes using anti-human c-MPL mouse monoclonal antibody in 1998. For the first two years, fundamental technique of post-embedding gold colloid method 〓sing immuno-electron microscopy was much learned, while in vitro culture of megakaryocytes isolated from bone marrow was continued as already reported to obtain the cell sample for c-MPL study. In conclusion, we failed in demonstrating the location of c-MPL by electro-microscopy, while some ultrastructure of the megakaryocytes were proved to be essential for cell differentiation leading to the production of proplatelets or platelets.

(4 results)