1999 Fiscal Year Final Research Report Summary
Analysis of cell cycle modulating proteins in the process of polyploidization of megakaryocytes.
| Project/Area Number |
10670970
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | TOKYO WOMEN'S MEDICAL UNIVERSITY |
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Principal Investigator |
TERAMURA Masanao Tokyo Women's Medical University, Department of Hematology, Lecturer, 医学部, 講師 (40188686)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shoko Tokyo Women's Medical University, Department of Hematology, Instructor, 医学部, 助手 (80256528)
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| Project Period (FY) |
1998 – 1999
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| Keywords | megakaryocyte / polyploidization / cdc2 / cyclin B1 / NF-E2 |
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| Research Abstract |
Mechanism of polypoidization of megakaryocyte remain largely unclarified. To clarify them further, we used a human megakaryoblastic cell line, Meg-J, which showed prominent polyploidization and augmented platelet glycoprotein (GP) Ib expression after incubation with K252a (an indolocarbasole derivative). In the process of K252a-induced polyploidizaton, levels of both cdc2 and cyclin B1 were elevated transiently and subsequently decreased. This suggests that the polyploidization process in Meg-J cells is at least in part associated with a transient elevation and subsequent decrease in the expression of cdc2/cyclin B1 complex. Next, we analyzed the expression of the transcription factors and observed that the expression of NF-E2 p45, but not those of GATA-1, GATA-2, Tal-1/SCL, Evi-1, and MafK, was increased after TPO and K252a stimulation. Gel-shift assay confirmed the enhanced binding activity to the NF-E2 site. The abolishment of NF-E2 p45 with NF-E2 antisense oligomers inhibited TPO plus K252a-induced polyploidization. These findings suggest that NF-E2 p45 is essential for the polyploidization of megakaryocytic cells.
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Research Products
(4 results)
