REGULATORY MECHANISM OF Cl-CHANNEL BY plCln.

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 10670983
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: AKITA UNIVERSITY
• Principal Investigator: TASHIMA Yohtalou School of Medicine, Akita University, Professor, 医学部, 教授 (30049796)
• Project Period (FY): 1998 – 1999
• Keywords: plCln / low osmolality / actin / E.Coli

Research Abstract

(1) E.Coli cells expressing mammalian plCln showed a significant increase in the resistance against a low environmental osmolality.
(2) Only 46-amino acid sequence included in the plCln molecule was enough for the acquired resistance with E.Coli cells.
(3) plCln in PPL-K1 cells (originated from pig kidneys) was moved and spread into cytosol from perinuclear region by the exposure of the cells to a low environmental osmolality.
(4) plCln in skeletal muscles was presented in a homologous form of hetero-oligomer with a constant length of actin-oligomer.

Research Products

• [Publications] Tao GZ.,Kobayashi A.,Itoh H.,Tashima Y.: "Expression of pICln in Escherichia Coli gives a strong tolerance to hypotonic stress"FEBS Letters. 434. 28-32 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Li Y.,Tao GZ.,Nagasawa H.,Tazawa H.,Kobayashi A.,Itoh H.,Tashima Y.: "pICln and cytosolic F-actin constitute a heteromeric complex with a constant molecular mass in rat skeletal muscles"J. Biochem. 126. 643-649 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ma YZ.,Tao GZ.,Kobayashi A.,Itoh H.,Tashima Y.,Kojima N.,Sendo M.: "Translocation of pICln in a pig kidney cell line, LLC-PKI, by low osmotic stress"Clin. Exp. Nephrol.. 3. 163-168 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tao,G-Z., Kobayashi, A., Itoh,H., Tashima,Y.: "Expression of plCln in Escherichia Coli gives a strong tolerance to hypotonic stress."FEBS Letters. 434. 28-32 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Li,Y., Tao,G-Z., Nagasawa, H., Tazawa, H., Kobayashi, A., Itoh, H., Tashima, Y.: "plCln and cytosolic F-action constitute a heteromeric complex with a constant molecuylar mass in rat skeletal muscles."J. Biochem.. 126. 643-649 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ma,Y-Z., Tao, G-Z., Kobayashi, A., Itoh, H., Tashima, Y., Kojima, N., Senoo, H.: "Translocation of plCln in a pig kidney cell line, LLC-PK1, by low osmotic stress."Clin. Exp. Nephrol.. 3. 163-168 (1999)
  • Description: 「研究成果報告書概要(欧文)」より