1999 Fiscal Year Final Research Report Summary

Cloning of Ca permeable ion channel in he kidney.

Research Project

Project/Area Number	10671002
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Kidney internal medicine
Research Institution	Jichi Medical School
Principal Investigator	SUZUKI Makoto JICHI Medical School, ACCOCIATED PROFESSOR, 医学部, 助教授 (10196868)
Project Period (FY)	1998 – 1999
Keywords	Ionchannel / Ca / Na
Research Abstract	To investigate molecules contributing Ca transport in the kidney, Isolation and in vitro expression of cDNAs was designed to be the subject of this study. By homology of Trp families, we isolated 4 different families; spliced variants of Trps, valiant of vanilloid receptors and its family, and Epithelial Ca channel family. Variant of vanilloid receptor has been unique mechanosensitive channel where stretch diminished the activity of this channel. Trp variant was further unique in expression, since original clone has not been expressed the Xenopus oocytes system we used. Ca permeable channels are compatible with our aims, although the cloning reported by others independently. We could express two clones of this family and find important channels in Ca homeostasis in the body.