1999 Fiscal Year Final Research Report Summary
Role of NKT cells in the acceptance of islet xenograftsin mice treated with anti-CD4 monoclonal antibody
| Project/Area Number |
10671145
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
YASUNAMI Yohichi Fukuoka Univ., Sch. Medicine, Assoc. Prof., 医学部, 助教授 (00166521)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
白石 君男 福岡大学, 医学部, 助手 (90187518)
加藤 寿彦 福岡大学, 医学部, 教授 (80078766)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | Islet transplantation / NKT cells / Rejection / Tolerance / xenotransplantation / Anti-CD4 antibody |
|---|
| Research Abstract |
Pancreatic islet transplantation represents a potential treatment for insulin-dependent diabetes mellitus. However. the precise cellular and molecular mechanisms of the immune reactions against allogeneic and xenogeneic transplanted islets remain nuclear. In the present study, we demonstrate that CD4a+ Vα l4 NKT cells, a distinct lymphoid cell lineage recently identified, are required for the acceptance of intrahepatic rat islet xenografts. Rat islet xenografts were accepted by C57BL/6 mice when certain doses of anti-CD4mAb were administrated after transplantation. No immunosuppressive drug is required in this procedure. The dose or anti-CD4mAb was critical, and the beneficial effect appeared to be associated with reappearance of CD4 + NKT cells around 14 days after transplantation. The anti-CD4mAb-mediated acceptance of rat islet xenografts was not observed in Vα 14 NKT cell-deficient mice, where essentially no Vα 14 NKT cells exist and conventional lymphoid cells remain intact. Moreover, the adoptive transfer of Vα 14 NKT cells into Vα 14 NKT cell-deficient mice reconstituted acceptance of rat islet xenografts. These results indicate that Vα 14 NKT cells play a crucial role in the acceptance of rat islet xenografts in mice treated with anti-CD4 antibody.
|
