2000 Fiscal Year Final Research Report Summary
A study of calcification mechanism of arteriosclerosis
| Project/Area Number |
10671718
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
YAMAMOTO Hirotsugu Nihon University, School of Dentistry at Matsudo, Department of Pathology, Professor, 松戸歯学部, 教授 (00102591)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANEDA Takashi Nihon University, School of Dentistry at Matsudo, Department of Radiology, Professor, 松戸歯学部, 教授 (40185947)
UTSUNOMIYA Tadahiko Nihon University, School of Dentistry at Matsudo, Department of Pathology, Research Assistant, 松戸歯学部, 助手 (50297850)
OAKADA Hiroyuki Nihon University, School of Dentistry at Matsudo, Department of Pathology, Lecturer, 松戸歯学部, 講師 (70256890)
MISHIMA Hiroyuki Nihon University, School of Dentistry at Matsudo, Department of Anatomy, Lecturer, 松戸歯学部, 講師 (30112957)
SAKAE Toshiro Nihon University, School of Dentistry at Matsudo, Department of Anatomy, Associate Professor, 松戸歯学部, 助教授 (20112948)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Keywords | arteriosclerosis / crystal / calcification / histopathology / FT-IR / oxalate calcium / phosphate calcium / SEM |
|---|
| Research Abstract |
Arteriosclerosis which consisted of lipid pigmentation, atheroma and calcification, is mainly occurred in the tunica intima. Although many studies concerning about pathogenesis of the lesion have been reported, the study of crystal formation has not been done extensively and conclusively, as yet. Therefore, the purpose of this study was to investigate the morphologic feature of the crystal in the arteriosclerosis from histopathological and biomineralogical viewpoints, and the following results were obtained.
Histopathologically, the arteriosclerosis was composed of fibrous hypertrophy of the tunica intima with formation of atheroma, ulcer and calcification. From the findings using a polarization microscopy several pieces of crystal structure, which showed with high birefringence, were scattered in the area of arteriosclerosis. The morphological features indicated a bundle rosette, or fused appearance of the crystals. In addition, the crystals were negative or dyed black with von Kossa staining.
From the analysis using Fourier transform infrared spectroscopy and scanning electron microscopy, the component of the crystal was thought to be calcium phosphate and/or calcium oxalate. The pathogenesis may have a relationship of oxalosis due to intestinal disorders or infection of major parasite.
|
