1999 Fiscal Year Final Research Report Summary
Studies on the matrix resicles of the odontoblast layer and cementoblast layer of bovine tooth germs.
| Project/Area Number |
10671945
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
MAKI Kenshi Kyushu Dental College, Professor, 歯学部, 講師 (60209400)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Mitsutaka Kyushu Dental College, Lecturer, 歯学部, 教授 (70047801)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Matrix resicles / Odontoblast / Alkaline phosphatase / Lactate dehydrogenase / metallo protease |
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| Research Abstract |
Matrix vesicles are extracelluar organelles that initiate mineralization in the extracelluler matrix of various calcifing tissues. The dentin was removed from bovine tooth germs. The matrix vesicle fractions, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation. Alkaline phosphatase activity(ALP) , protease activity, and lactate dehydrogenase activity (LDH) were assayed for the marker enzyme of matrix vesicles.
1. There were not only the matrix vesicle but also matrix vesicle-like-vesicle with a high density in the dentin, the vesicle contained ALP and LDH but did not protease.
2. Protease activity after inhibition with o-phenanthroline was restored by the addition of CoィイD12+ィエD1 and ZnィイD12+ィエD1, These results show that the protese in the matrix vesicles of the odontoblasts and predentin is a nietalloprotese.
3. Aldolase, aspartate : 2-oxoglutarate aminotransferase and alanine : 2-oxoglutarate aminotransferase,cytosonic enzymes except LDH ,were not detected in these vesicles. These results suggest the presence of a mechanism for the specific uptake of cytosolic LDH.
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