1999 Fiscal Year Final Research Report Summary
Functional studies on lymphocytes with OKT4 epitope deficiency
| Project/Area Number |
10672182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Wakayama Medical College |
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Principal Investigator |
TAKENAKA Toru Wakayama Medical College, Laboratory Medicine, Associate Professor, 医学部, 助教授 (00137259)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMINE Hirokazu Wakayama Medical College, Laboratory Medicine, Instructor, 医学部, 講師 (70155810)
KURIBAYASHI Koichi Wakayama Medical College, Laboratory Medicine, Instructor, 医学部, 講師 (00192039)
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| Project Period (FY) |
1998 – 1999
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| Keywords | OKT4 epitope deficiency / homozygote and heterozygote / lymphocyte response to PPD or anti-CD3 monoclonal antibody / Examination of DNA synthesis by MTT assay / Detection of mRNAs for IL-2 IFN-γ, IL-4 and IL-5 |
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| Research Abstract |
We have demonstrated that OKT4 epitope deficiency was responsible for one point mutation of the gene encoding 240 amino acid, which is substituted CGG to TGG, and that the frequency of complete and incomplete OKT4 epitope deficiency, i.e. homozygote and heterozygote, in Japanese was 0.5 and 12.8%, respectively (Takenaka T., et al. : J. Immunol., 1993).
In the present studies, functional studies of lymphocytes were performed in 2 cases of homozygote, 2 cases of heterozygote, and 4 healthy individuals as age-matched control. Purified-protein derivatives of mycobacterium tuberculosis (PPD) and ante-CD3 monoclonal antibody (αCD3 mAb) of solid phase was used for lymphocyte stimulation. The reason why? PPD induces the lymphocyte response via class II MHC molecule plus PPD-derived peptide processed by antigen-presenting cells. On the other hand, αCD3 mAb directly react to T-cell receptor/CD3 molecule complex, which induces lymphocyte response. After stimulated lymphocytes by different way above described, DNA synthesis and expression of mRNAs for IL-2, IFN-γ, IL-4 and IL-5 were examined by MTT assay and RT-PCR, respectively. Consequently, there is no difference in DNA synthesis of the lymphocytes among homozygotes, heterozygotes, and healthy individuals. Furthermore, the difference was not shown in the expression of mRNAs for IL-2, IFN-γ, IL-4 and IL-5.
On the basis of these experiments, it is considerable that there are no functional abnormalities in thelymphocytes of complete and incomplete OKT4 epitope deficiency.
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Research Products
(3 results)
