1999 Fiscal Year Final Research Report Summary
Direct Hemoperfusion with Polymyxin B Immobilized Fiber (PMX) in Acute Lung Injury Induced by Endotoxemia in Anesthetized Sheep
| Project/Area Number |
10672261
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
HONDA Takayuki School of Medicine, Shinshu University, Associate Professor, 医学部, 助教授 (80238815)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Keisaku University Hospital, Shinshu University, Assistant, 医学部附属病院, 助手 (70242691)
芝本 利重 信州大学, 医学部, 助教授 (90178921)
KUBO Keishi School of Medicine, Shinshu University, Professor, 医学部, 教授 (80143965)
KOIZUMI Tomonobu University Hospital, Shinshu University, Assistant, 医学部附属病院, 助手 (20273097)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | Acute lung injury / Acute respiratory distress syndrome / Endotoxemia / anesthetized sheep / Lung lymph fistula / Polymyxin B Immobilized Fiber (PMX) / Shock / Respiratory failure |
|---|
| Research Abstract |
We have performed the following 2 projects under the grant.
(1) In anesthetized sheep with lung lymph fistula and systemic and pulmonary hemodynamic monitoring, we evaluated the effect of direct hemoperfusion with Polymyxin B immobilized fiber (PMX) in acute lung injury (ALI) induced by E. coli endotoxemia (10 μg/kg). Endotoxemia produced shock (decreased systemic blood pressure), pulmonary hypertension, circulating leukopenia and hypoxemia during the early phase after endotoxcmia and the increases in lung lymph flow, suggesting permeability edema, during the late phase. Direct hemoperfusion (60ml/hr) with PMX significantly improved the shock and hypoxemia induced by endotoxemia, though it did not changes the time course of leukopenia, pulmonary hypertension and lung lymph dynamics.
(2) We tried to treat 8 patients with ALI and acute respiratory distress syndrome (ARDS) using direct hemoperfusion with PMX. After 2 times of the treatment, systolic systemic pressure (from 105 ± 8 mmHg to 137 ± 5), diastolic pressure (from 63 ± 10 mmHg to 78 ± 7 mmHg) and PaO2/FiO2 (P/F ratio) (from 130 ± 20 to 170 ± 15) were significantly improved.
|
