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2002 Fiscal Year Final Research Report Summary

Supervision of the Research of Single-cell Molecular Technology

Research Project

Project/Area Number 11227101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo University of Agriculture and Technology, Faculty of Technology, Professor, 工学部, 教授 (10143653)

Co-Investigator(Kenkyū-buntansha) HORIKOSHI Koki  Toyo University, Department of Life Science, Professor, 生命科学部, 教授 (80087551)
KAWATA Satoshi  Osaka University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (30144439)
IMACHI Ko  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10010076)
NAGAI Kazuo  Chubu University, Department of Applied Biology, Professor, 応用生物学部, 教授 (00011974)
MATSUE Tomokazu  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (70173797)
ANDO Joji  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20159528)
WATANABE Kimitsuna  The University of Tokyo, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (00134502)
AIZAWA Masuo  Tokyo Institute of Technology, President, 学長 (00016742)
Project Period (FY) 1999 – 2002
Keywordssingle-cell / cell function analysis / viability imaging / environmental signal responsive cells
Research Abstract

This study started in October 1, 1999 and ended in March 31, 2003. In the former half period (1999.10-2001.3), the supervisor group encouraged every investigator to challenge new research subjects focused on the single-cell molecular technology. In the latter half period (2001.4-2003.3), priority was given to several subjects that were regarded as typical single-cell research subjects. They were, for example, "The development of a single-cell experiment system using single-cell manipulation supporting robot" (A-group), "Single-cell bio-imaging using an electrochemical scanning microscope" (B-group), and "The construction of novel plasimids composed of stress response genes and a fluorescent protein reporter gene" (C-group). The expected results of these research subjects were thought to be the important basis of future developmental study on the creation of cells, tissues, and animals with novel functions. In order to discuss about such a prospect, a symposium entitled "From cells to the creation of tissues" was held on November 11-12, 2002. In summary, many experimental know-hows and study results on single-cells were accumulated. Their contribution to biotechnology and bioscience was remarkably great.

  • Research Products

    (194 results)

All Other

All Publications (194 results)

  • [Publications] H.Matsuoka: "Control of the Cross Membrane Potential of a Cultured Tobacco Cell and Simultaneous Measurement of the Electrical Impedance of Its Membrane."Electrochemistry. 67(1). 18-21 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "The effect of flow on the neutrophil-mediated Ca^<2+> responses In human vascular endothelial cells stimulated by endotoxin."Jpn.J.Surg.. 29. 966-969 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Control of the Quantity and the Timing of Microinjection into a Bio-Cell with a Multifunctional Microelectrode."Electrochim.Acta. 44. 3801-3807 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Effects of a Pulsing Electric Signal on the Cross Membrane Potential and the Cell Division Potentiality of a Single Cell of Tobacco."Bioelectrochem.Bioenerg. 49. 65-72 (1999)

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  • [Publications] T.Matsue: "Dual-Imaging of Topography and Photosynthetic Activity of a Single Protoplast by Scanning Electrochemical Microscopy."Anal.Chem.. 71. 4637-4641 (1999)

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  • [Publications] M.Aizawa: "Electrically stimulated modulation of cellular function in proliferation, differentiation, and gene expression."Electrochemistry. 67. 118-125 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Nagai: "Osteoblastic cells induce fusion and activation of osteoclasts through a mechanism independent of macrophage-colony-stimulating factor production."Cell Tissue Res.. 298. 324-334 (1999)

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  • [Publications] T.Matsue: "Chemiluminescence Imaging of Localized Enzymes by Scanning Chemical Reaction Microscopy."Chem.Lett.. 200-201 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Imaging of Diaphorase Activity Micropatterned at Gold Arrays with Scanning Electrochemical Microscopy."Chem.Lett.. 458-459 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "(Ba-16) Patterning and Characterization of Surfaces with Organic and Biological Molecules by the Scanning Electrochemical Microscopy (SECM)."Anal.Chem.. 71. 3431-3435 (2000)

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  • [Publications] T.Matsue: "Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy."Electrochemistry. 68. 890-892 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Immunoassay of MRSA-related Toxic Protein, Leukocidin, with Scanning Electrochemical Microscopy."Anal.Chem.. 72. 5761-5765 (2000)

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  • [Publications] H.Matsuoka: "Elicitor Action via Cell Membrane of a Cultured Rice Cell Demonstrated by the Single-Cell Transient Assay."J.Biotechnol.. 76. 227-232 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "pH-Microelectrode-micropipette system for the measurement of Enzyme Reactions in Picoliter Space of a Living Plant Cell."Anal.Chim.Acta. 404(2). 223-229 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Modulation of Ca^<2+> Influx into a Single Rice Cell with an Electric Signal."Electrochemistry. 68. 333-336 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Measurement of Glucose Uptake and Intracellular Calcium Concentration in Single, Living Pancreatic β-Cells."J.Biol.Chem.. 275. 22278-22283 (2000)

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  • [Publications] H.Matsuoka: "Distinctive Effects of Menadione on the In Vivo Catalase Activity and the Adaptation to Hydrogen Peroxide Stress in Yeast Cells."Biocontrol Sci.. 5. 103-106 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Use of Acetylene for the Fabrication of a Glass Capillary Carbon Microelectrode."Electrochemistry. 68(11). 924-926 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Fluid shear stress activates Ca^<2+> influx into human endothelial cells via P2X4 purinoceutors."Circ.Res.. 87. 385-391 (2000)

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  • [Publications] J.Ando: "P2X4 receptors mediate ATP-induced calcium influx in human vascular endothelial cells."Am.J.Physiol Heart Circ.Physiol.. 279. H285-H292 (2000)

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  • [Publications] K.Nagai: "Intracellular calcium and protein kinase C mediate expression of receptor activator of nuclear factor-B ligand and osteoprotegerin in osteoclasts."Endocrinology. 141. 4711-4719 (2000)

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  • [Publications] K.Nagai: "Compactin suppresses bone resorption by inhibiting the fusion of prefusion osteoclasts and disrupting the actin ring in osteoclasts."J.Bone Miner.Res.. 15. 650-662 (2000)

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  • [Publications] K.Nagai: "RANKL and IL-1 induce the fusion of mononuclear preosteoclasts into multinucleated osteoclasts through their receptors by activating NF-kappaB."Cytotechnology. 33. 203-211 (2000)

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  • [Publications] M.Aizawa: "Electrically Stimulated Induction of hsp70 Gene Expression in Mouse Astroglia and Fibrobrast Cells."J.Biotechnol. 79(1). 53-61 (2000)

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  • [Publications] M.Aizawa: "Osteogenesis Coordinated in Stromal Stem Cells by Adipogenesis Dependent BMP-2 Expression System."Tissue Engineering. 6(1). 9-18 (2000)

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  • [Publications] H.Matsuoka: "Purification and Characterization of an Autoregulatory Substance Capable of Regulating the Morphological Transition in Candida albicans."Proc.Natl.Acad.Sci.USA. 98. 4664-4668 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Detection of Antifungal Activity in Belamcanda chinensis by a Sibgle-cell Bioassay Method and Isolation of Its Compound, Tectorigenin.Biosci."Biotechnol.Biochem.. 65. 939-942 (2001)

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  • [Publications] M.Aizawa: "Bioelectronic evolution in developing molecular and cellular biodevices."Electrochemistry. 69. 921-928 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Sp1-mediated downregulation of P2X4 receptor gene transcription in endothelial cells exposed to shear stress."Am.J.Physiol Heart Circ.Physiol.. 280. H2214-H2221 (2001)

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  • [Publications] J.Ando: "Microvascular and interstitial PO2 measurements in rat skeletal muscle by phophorescence quenching."J.Appl.Physiol.. 91. 321-327 (2001)

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  • [Publications] J.Ando: "mRNA expression on shape-engineered endothelial cells : Adhesion molecules ICAM-1 and VCAM-1."J.Biomed.Mater.Res.. 54. 366-372 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Upregulatory expression of form and Transforming growth factor-□ by fluid shear stress in vascular endothelial cells."Arterioscler Thromb Vasc Biol.. 21. 785-790 (2001)

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  • [Publications] T.Matsue: "Oxygen Consumption of Single Bovine Embryos Probed with Scanning Electrochemical Microscopy."Anal.Chem.. 73. 3751-3759 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Dielectrophoretic Manipulation of a Single Cell with Dual-Microdisk Electrode."Bioelectrochemistry. 54. 33-37 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "A Microbial-Chip Combined with Scanning Electrochemical Microscopy."Biotech.Bioeng. 76. 391-394 (2001)

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  • [Publications] T.Matsue: "Mapping Peroxidase in Plant Tissues by Scanning Electrochemical Microscopy."Bioelectrochemistry. 54. 151-156 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Microspots of GOD-HRP Bienzyme for Scanning Chemisluminescence Microscopy with Higher Resolution."Electrochemistry. 69. 946-948 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Nagai: "The effects of retinoic acid on reversing the adipocyte differentiation into an osteoblastic tendency."Cytotechnology. 36. 125-136 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] M.Aizawa: "Construction of the Cellular Biosensing System for Assessment of Drugs for the Nervous System."Chemical Sensors, Supplement A. 17. 169-171 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] M.Aizawa: "Bioelectronic evolution in developing molecular and cellular biodevices."Electrochemistry. 69. 921-928 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "In vivo model for visualizing flap microcirculatin of ischemia-reperfusion."Micorsurgery. 22. 304-310 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Sites of Ca^<2+> wave initiation move with caveolae to the trailing edge of migrating cells."J.Cell Sci.. 115. 475-484 (2002)

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  • [Publications] J.Ando: "Dorsal bipedicled island skin flap : a new flap model in mice."Scand J Plast Reconstr Surg Hand Surg.. 36. 262-267 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Rapid Viability Assessment of Yeast cells Using Vital Staining with 2-NIBDG, a Fluorescent Derivative of Glucose."Intern.J.Food Microbiol.. 76. 47-53 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Simultaneous Imaging of Multiple Fluorescent Probes in Bio-Cells."Anal.Sci.. 18. 1321-1324 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Single-Cell Imaging of Ca^<2+> Influx into Bovine Endotherial Cells Occurring in Response to an Alternating Electric Stimulus."Anal. Sci.. 18. 1205-1208 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Rapid Detection of Small Number of Viable Cells by Fluorescent Glucose Method."J.Food Safety. 22. 141-153 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Single-cell Viability Assessment with a Novel Spectro-imaging System."J.Biotechnol.. 94. 299-308 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Simultaneous Detection of Uric Acid and Glucose on a Dual-Enzyme Chip Using Scanning Electrochemical Microscopy/Scanning Chemiluminescence Microscopy."Anal.Chim.Acta.. 1-8. 263-270 (2002)

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  • [Publications] T.Matsue: "Micropatterning of HeLa Cells on Glass Substrates and Evaluation of Respiratory Activity using Microelectrodes."Langmuir. 18. 3645-3649 (2002)

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  • [Publications] T.Matsue: "Micropatterned HeLa Cell Culture on PEG Monolayer-Coated Glass Substrates."Chem.Lett.. 9. 904-905 (2002)

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  • [Publications] T.Matsue: "Electroanalysis of Metabolic Flux from Single Cells in Picolitre-Volume Microsystems."Anal.Chem.. 74. 5001-5008 (2002)

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  • [Publications] K.Nagai: "Fenton reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death."Biochem Biophys Res Commun.. 292. 94-101 (2002)

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  • [Publications] K.Nagai: "Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1 and RANKL."J.Cell Physiol, J.Cell Physiol.. 190. 101-108 (2002)

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  • [Publications] M.Aizawa: "Thermostabiization of protein A-luciferase fusion protein by single amino acid mutation."Biotech.Lett.. 147-149. 24 (2002)

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  • [Publications] M.Aizawa: "Cellular biosensing system for discovery of protein synthesis inhibitor with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae."Biosens.Bioelect.. 17. 209-215 (2002)

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  • [Publications] M.Aizawa: "Non-destructive monitoring of rpoS promotor activity as stress marker for evaluating cellular physiological status."J.Biotechnol.. 95. 85-93 (2002)

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  • [Publications] M.Aizawa: "Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp."Biotech.Lett.. 24. 269-273 (2002)

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  • [Publications] M.Aizawa: "Pressure sensitivity of HSP7O promoter in transformed 3T3-L1 cells."JAMSTEC J.Deep Sea Res.. 20. 53-58 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Matsuoka: "Dielectrophoretic Selection of Viable Single-cells of Rice and Tobacco."Electrochemistry. 71. 446-448 (2003)

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  • [Publications] H.Matsuoka: "Isolation of H^+-translocating ATPase in Tonoplast of Tradescantia virginiana L.Leaf Cells."J.Biotechnol. 100. 221-229 (2003)

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  • [Publications] H.Matsuoka: "Expression of Rice Chitinase Gene Triggered by the Direct Injection of Ca^<2+>."J.Biotechnology. (in press).

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Electrochemical / Photochemical Formation of Enzyme Patterns on Glass Substrates using a Scanning Electrochemical / Confocal Microscope."Sens.Actuat.B. (in press).

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  • [Publications] T.Matsue: "Imaging of Immobibzed Enzyme Spots by Scanning Chemiluminescence Microscopy with Electrophoretic Injection."Biosens.Bioelectron.. (in press).

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      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Intercellular Ca^<2+> Imaging for Micropatterned Cardiac Myocytes."Biotech.Bioeng.. (accepted).

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  • [Publications] 安藤譲二: "Shear stressに対する細胞応答"医学のあゆみ. 191巻. 357-362 (1999)

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  • [Publications] 安藤譲二: "ずり応力と内皮細胞の遺伝子発現調節"細胞工学. 18巻. 182-187 (1999)

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  • [Publications] H.Matsuoka: "Microbioelectronics for Single-Cell Experiment."Electrochemistry. 68. 314-320 (2000)

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  • [Publications] 安藤譲二: "メカニカルストレスと血管医学"血管医学. 1巻. 31-37 (2000)

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  • [Publications] 安藤譲二: "メカニカルストレスと血管"日本老年医学会雑誌. 37巻. 666-675 (2000)

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  • [Publications] 安藤譲二: "生体テンセグリティ:新しい構造形成原理"日本ME学会誌. 14巻. 11-16 (2000)

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  • [Publications] 安藤譲二: "Shear stressと動脈硬化"医学のあゆみ. 193巻. 318-322 (2000)

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  • [Publications] 安藤譲二: "ずり応力と内皮細胞"治療学. 34巻. 29-32 (2000)

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  • [Publications] 安藤譲二: "血行力学からみた大動脈瘤形成機序"Cardiac Practice. 11巻. 27-33 (2000)

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  • [Publications] 安藤譲二: "内皮細胞のシェアストレス感知機構"医学のあゆみ. 192巻. 1217-1221 (2000)

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  • [Publications] 安藤譲二: "シェアストレス"日本臨床. 58巻. 330-334 (2000)

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  • [Publications] 相澤益男: "電気刺激による細胞機能制御の機構."化学と生物. 38(8). 503-507 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] 相澤益男: "動物細胞への電気刺激とストレス応答タンパク質の発現"バイオサイエンスとバイオインダストリー. 59(2). 110-111 (2001)

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  • [Publications] 松岡英明: "単一細胞実験技術の開発"バイオサイエンスとバイオインダストリ. 59(7). 31-34 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "単一細胞実験法による遺伝子発現解析"生物物理. 41(5). 255-257 (2001)

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  • [Publications] 安藤譲二: "血管内皮細胞の血流刺激受容と遺伝子応答"Molecular Medicine. 38巻. 994-999 (2001)

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  • [Publications] 安藤譲二: "内皮細胞のずり応力センサー機構"Molecular Medicine. 38巻. 36-40 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "メカニカルストレスによる血管機能制御"THERAPEUTIC RESEARCH. 22巻. 2657-2664 (2001)

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  • [Publications] 安藤譲二: "メカニカルストレスと血管リモデリング"現代医療. 33巻. 67-72 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管内皮細胞の機械受容・応答機構"生体材料. 19. 103-110 (2001)

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  • [Publications] 珠玖 仁, 大矢博昭, 末永智一: "走査型電気化学顕微鏡"Electrochiemistry. 69. 806-810 (2001)

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  • [Publications] 末永智一: "マイクロ電極を用いたバイオセンシングとイメージング"未来材料. 1. 6-11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "実用化迫る抗体チップ-マイクロ電極による検出システム"免疫化学測定法研究会年報. 4. 63-68 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永井和夫: "生理活性物質を用いた骨代謝細胞の分化と機能発現の解析"日本農芸化学会誌. 75. 111-119 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "キャピラリーカーボンマイクロ電極"マテリアルインテグレーション. 15(2). 45-49 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管内皮細胞における流れずり応力のカルシウム・シグナリング"日本バイオレオロジー学会誌. 16巻. 3-7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "新しいスペクトロイメージングシステムによる単一細胞の顕微分光"telecom FRONTIER. No.36. 23-29 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "植物用遺伝子導入支援ロボットを目指すテクノロジー"農業低温科学研究情報. 9(2). 2-4 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血流による血管内皮機能の調節"現代医療. 34巻. 2714-2719 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管構築のシステムバイオロジー"血管医学. 3巻. 511-517 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管のシステムバイオロジー"血管医学. 3巻. 445-448 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "メカニカルストレスの血管への作用"日本血栓止血学会誌. 13巻. 227-236 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "内皮細胞のメカニカルストレス感知・応答機構"実験医学. 20巻. 142-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "メカノバイオロジーからみた生体構造形成原理"BME. 16巻. 22-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Flow-induced endothelial gene regulation. In"Mechanical Forces and the Endothelium", P.I.Lelkes ed."Harwood Academic Publishers, London. 111-126 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "Shear stress.動脈硬化の最前線 北徹編"羊土社. 131-141 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "植物を利用するセンサ技術、"バイオミメティックハンドブック"(長田義仁編)"エヌ・ティー・エス. 1038-1043 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "生体膜の電気化学(Electrochemistry of Biomembrane)"電気化学便覧 第5版"(電気化学会編)"丸善. 332-335 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Ando: "Shear Stress-Dependent Regulation of Endothelial Cell Functions. In"New Frontier in Vascular Biology ; Thrombosis & Hemostasis"K.Suzuki et al.eds"Eibun Press, Osaka. 199-215 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管内皮細胞。基礎生化学実験法2 生体試料"東京化学同人. 58-61 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "シェアストレス。最新・分子動脈硬化学 森崎信博ら編"メディカルレビュウ社. 233-240 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "電気化学顕微鏡の基礎と応用表面技術,51"46-52 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T Matsue: "Characterization and Imaging of Single Cells with Scanning Electrochemical Microscopy Electroanalysis,12"653-659 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Microamperometric Sensing of Localized Biomolecules in"Sensors Update",Ed.By H.Baltes, W.Gopel, J.Hesse, Wiley-VCH, Chapter12."231-242 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "(Bf-10)生体材料工学(熊谷 泉,金谷茂則編)第8章,"生命工学""共立出版. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 相澤益男: "動物細胞工学ハンドブック(分担)、電気による制御"朝倉書店. 91-92 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 相澤益男: "細胞制御技術.第5版 電気化学便覧"丸善. 338-340 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 相澤益男: "電気による制御.動物細胞工学ハンドブック"朝倉書店. 91-92 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "血管内皮細胞のバイオミメティクス バイオミメティクスハンドブック 長田義仁編"エヌ・ティ・エス. 828-838 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nagai: "Escherichia coli ribonuclease G.In A.W.Nicholson(ed.),Methods in Enzymology, vol.342,"Ribonucleases""Academic Press, Inc.,NewYork.. 55-63 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "バイオセンシング・バイオエレクトロニクス(Biosensing・Bioelectronics)、"生命工学への招待-基礎と応用-"(松永是編)"朝倉書店. 79-82 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "「4章.微生物制御法の原理と科学」の中の「4.1.物理的方法」、「5章.制御における測定法・評価法」、「8章.菌株保存」"微生物制御の科学と工学'(土戸哲明、高麗寛紀、松岡英明、小泉淳一、著)"講談社サイエンティフィク. 44-54,82-95,130-136 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤譲二: "動脈硬化にかかわる機械因子のしくみは?第16回「大学と科学」公開シンポジウム2101012 講演収録集動脈硬化のしくみをさぐる"クワブロ社. 97-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "顕微・形態分析"機器分析実験"(梅沢喜夫,本水昌二,渡会 仁,寺前紀夫編).第8章"東京化学同人. 12387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松岡英明: "マイクロインジェクションを軽労化する単一細胞実験システムの構築 平成14年度マイクロバイオリアクターシステムの構築に関する基礎調査委託事業報告書"農林水産技術情報協会. 46-59 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Matsue: "Intercellular Ca^<2+> Imaging for Micropatterned Cardiac Myocytes."Biotech. Bioeng. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Electrochemical/Photochemical Formation of Enzyme Patterns on Glass Substrates using a Scanning Electrochemical/Confocal Microscope."Biosens. Bioelectron. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Imaging of Immobilized Enzyme Spots by Scanning Chemiluminescence Microscopy with Electrophoretic Injection."Biosens. Bioelectron. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Scanning Electrochemical Microscopy Applied to Biological Systems in "Encyclopedia of Electrochemistry. Vol. 9. Bioelectrochemistry", Ed. By G. S. Wilson."Wiley-VCH, Chapter 10 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Expression of rice chitinase gene triggered by the direct injection of Ca^<2+>."J. Biotechnol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Dielectrophoretic Selection of Viable Single-cells of Rice and Tobacco."Electrochemistry. 71. 446-448 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Isolation of H^+-translocating ATPase in Tonoplast of Tradescantia virginiana L. Leaf Cells."J. Biotechnol.. 100. 221-229 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.matsuoka: "Simultaneous Imaging of Multiple Fluorescent Probes in Bio-Cells."Anal. Sci. 18. 1321-1324 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Single-Cell Imaging of Ca^<2+> Influx into Bovine Endotherial Cells Occurring in Response to an Alternating Electric Stimulus."Anal. Sci.. 18. 1205-1208 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Rapid Detection of Small Number of Viable Cells by Fluorescent Glucose Method."J. Food Safety. 22. 141-153 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Single-cell Viability Assessment with a Novel Spectro-imaging System."J. Biotechnol.. 94. 299-308 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Rapid Viability Assessment of Yeast cells Using Vital Staining with 2-NBDG, a Fluorescent Derivative of Glucose."Intern. J. Food Microbiol.. 76. 47-53 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "In vivo model for visualizing flap microcirculation of ischemia-reperfusion."Micorsurgery. 22. 304-310 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Sites of Ca^<2+> wave initiation move with caveloae to the trailing edge of migrating cells."J. Cell Sci.. 115. 475-484 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Dorsal bipedicled island skin flap : a new flap model in mice."Scand J Plast Reconstr Surg Hand Surg. 36. 262-267 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Simultaneous Detection of Uric Acid and Glucose on a Dual-Enzyme Chip Using Scanning Electrochemicl Microscopy/Scanning Chemiluminescence Microscopy."Anal. Chim. Acta. 1-8. 263-270 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Micropatterning of HeLa Cells on Glass Substrates and Evaluation of Respiratory Activity using Microelectrodes."Langmuir. 18. 3645-3649 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Micropatterned HeLa Cell Culture on PEG Monolayer-Coated Glass Substrates."Chem. Lett.. 9. 904-905 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Electroanalysis of Metabolic Flux from Single Cells in Picolitre-Volume Microsystems."Anal. Chem.. 74. 5001-5008 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Fenton Reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death."Biochem Biophys Res Commun. 292. 94-101 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1 and RANKL."J. Cell Physiol, J. Cell Physiol.. 190. 101-108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Thermostabilization of protein A-luciferase fusion protein by single amino acid mutation."Biotech. Lett.. 24. 147-149 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Cellular biosensing system for discoery of protein synthesis inhibitor with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae."Biosens. Bioelect.. 17. 209-215 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Non-destructive monitoring of rpoS promotor activity as stress marker for evaluating cellular physiological status."J. Biotechnol.. 95. 85-93 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp."Biotech. Lett.. 24. 269-273 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Pressure sensitivity of HSP70 promoter in transformed 3T3-L1 cells."JAMSTEC J. Deep Sea Res.. 20. 53-58 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Purification and Characterization of an Autoregulatory Substance Capable of Regulating the Morphological Transition in Candida albicans."Proc. Natl. Acad. Sci. USA. 98. 4664-4668 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Detection of Antifungal Activity in Belamcanda chinensis by a Sibgle-cell Bioassay Method and Isolation of Its Compound, Tectorigenin."Biosci. Biotechnol. Biochem.. 65. 939-942 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Sp1-mediated downregulation of P2X4 receptor gene transcription in endothelial cells exposed to shear stress."Am. J. Physiol Heart Circ. Physiol.. 280. H2214-H2221 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Microvascular and interstitial PO2 measurements in rat skeletal muscle by phophorescence quenching."J. Appl. Physiol.. 91. 321-327 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "mRNA expression on shape-engineered endothelial cells : Adhesion molecules ICAM-1 and VCAM-1."J. Biomed. Mater. Res.. 54. 366-372 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Upregulatory expression of furin and Transforming growth factor-□ by fluid shear stress in vascular endothelial cells."Arterioscler Thromb Vasc Biol.. 21. 785-790 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Oxygen Consumption of Single Bovine Embryos Probed with Scanning Electrochemical Microscopy."Anal. Chem.. 73. 3751-3759 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Dielectrophoretic Manipulation of a Single Cell with Dual-Microdisk Electrode."Bioelectrochemistry. 54. 33-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "A Microbial-Chip Combined with Scanning Electrochemical microscopy."Biotech. Bioeng.. 76. 391-394 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Mapping Peroxidase in Plant Tissues by Scanning Electrochemical Microscopy."Bioelectrochemistry. 54. 151-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Microspots of GOD-HRP Bienzyme for Scanning Chemisluminescence Microscopy with Higher Resolution."Electrochemistry. 69. 946-948 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "The effects of retinoic acid on reversing the adipocyte differentiation into an osteoblastic tendency."Cytotechnology. 36. 125-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Construction of the Cellular Biosensing System for Assessment of Drugs for the Nervous System."Chemical Sensors. 17,Supplement A. 169-171 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Escherichia coli ribonuclease G. In A. W. Nicholson (ed.)"Methods in Enzymology. vol.342. 55-63 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Bioelectronic evolution in developing molecular and cellular biodevices."Electrochemistry. 69. 921-928 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Intracellular calcium and protein kinase C mediate expression of receptor activator of nuclear factor-B ligand and osteoprotegerin in osteoclasts."Endocrinology. 141. 4711-4719 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Compactin suppresses bone resorption by inhibiting the fusion of prefusion osteoclasts and disrupting the actin ring in osteoclasts."J. Bone Miner. Res.. 15. 650-662 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "RANKL and IL-1 induce the fusion of mononuclear preosteoclasts into multinucleated osteoclasts through receptors by activating NF-kappaB."Cytotechnology. 33. 203-211 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Electrically Stimulated Induction of hsp70 Gene Expression in Mouse Astroglia and Fibrobrast Cells."J. Biotechnol.. 79(1). 53-61 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Osteogenesis Coordinated in Stromal Stem Cells by Adipogenesis Dependent BMP-2 Expression System."Tissue Engineering. 6(1). 9-18 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Microbioelectronics for Single-Cell Experiment."Electrochemistry. 68. 314-320 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Shear Stress-Dependent Regulation of Endothelial Cell Functions."New Frontier in Vascular Biology ; Thrombosis & Hemostasis. 199-215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Elicitor Action via Cell Membrane of a Cultured Rice Cell Demonstrated by the Single-Cell Transient Assay."J. Biotechnol.. 76. 227-232 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "pH-Microelectrode-micropipette system for the measurement of Enzyme Reactions in Picoliter Space of a Living Plant Cell."Anal. Chim. Acta. 404(2). 223-229 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Modulation of Ca^<2+> Influx into a Single Rice Cell with and Electric Signal."Electrochemistry. 68. 333-336 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Measurement of Glucose Uptake and Intracellular Calcium Concentration in Single, Living Pancreatic □-Cells."J. Biol. Chem.. 275. 22278-22283 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Distinctive Effects of Menadione on the In Vivo Catalase Activity and the Adaptation to Hydrogen Peroxide Stress in Yeast Cells."Biocontrol Sci.. 5. 103-106 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Use of Acetylene for the Fabrication of a Glass Capillary Carbon Microelectrode."Electrochemistry. 68(11). 924-926 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Fluid shear stress activates Ca^<2+> influx into human endothelial cells via P2X4 purinoceptors."Circ. Res.. 87. 385-391 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "P2X4 receptors mediate ATP-induced calcium influx in human vascular endothelial cells."Am. J. Physiol Heart Circ. Physiol.. 279. H285-H292 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Shear Stress-Dependent Regulation of Endothelial Cell Functions."In "New Frontier in Vascular Biology ; Thrombosis & Hemostasis". 199-215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Microbioelectronics for Single-Cell Experiment."Electrochemistry. 68. 314-320 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsue: "Chemiluminescence Imaging of Localized Enzymes by Scanning Chemical Reaction Microscopy."Chem. Lett.. 200-201 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Imaging of Diaphorase Activity Micropatterned at Gold Arrays with Scanning Electrochemical Microscopy."Chem. Lett.. 458-459 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Patterning and Characterization of Surfaces with Organic and Biological Molecules by the Scanning Electrochemical Microscopy (SECM)."Anal. Chem.. 71. 3431-3435 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy."Electrochemistry. 68. 890-892 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Immunoassay of MRSA-related Toxic Protein, Leukocidin, with Scanning Electrochemical Microscopy."Anal. Chem. 72. 5761-5765 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Characterization and Imaging of Single Cells with Scanning Electrochemical Microscopy."Electroanalysis. 12. 653-659 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Microamperometric Sensing of Localized Biomolecules."in "Sensors Update". 12. 231-242 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Control of the Quantity and the Timing of Microinjection into a Bio-Cell with a Multifunctional Microelectrode."Electrochim. Acta. 44. 3801-3807 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Effects of a Pulsing Electric Signal on the Cross Membrane Potential and the Cell Division Potentiality of a Single Cell of Tobacco."Bioelectrochem. Bioenerg.. 49. 65-72 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Matsuoka: "Control of the Cross Membrane Potential of a Cultured Tobacco Cell and Simultaneous Measurement of the Electrical Impedance of Its Membrane."Electrochemistry. 67(1). 18-21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "The effect of flow on the neutrophil-mediated Ca^<2+> responses In human vascular endothelial cells stimulated by endotoxin."Jpn. J. Surg.. 29. 966-969 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Flow-induced endothelial gene regulation. In "Mechanical Forces and the Endothelium", P.I.Lelkes ed."Harwood Academic Publishers, London. 111-126 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Ando: "Flow-induced endothelial gene regulation. Mechanical Forces and the Endothelium."Harwood Academic Publishers, London. 111-126 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Dual-Imaging of Topography and Photosynthetic Activity of a Single Protoplast by Scanning Electrochemical Microscopy."Anal. Chem.. 71. 4637-4641 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Nagai: "Osteoblastic cells induce fusion and activation of osteoclasts through a mechanism independent of macrophage-colony-stimulating factor production."Cell Tissue Res.. 298. 324-334 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aizawa: "Electrically stimulated modulation of cellular function in proliferation, differentiation, and gene expression."Electrochemistry. 67. 118-125 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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