2003 Fiscal Year Final Research Report Summary
Evolutaionary mechanism for the adaptive immune system as revealed by the structural analysis of the medaka major histocompatibility complex
| Project/Area Number |
11236205
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NONAKA Masaru The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40115259)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Shiga University of Medical Science, School of Medicine, Professor, 医学部, 教授 (00110560)
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| Project Period (FY) |
1999 – 2003
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| Keywords | MHC / BAC contig / Polymorphism / Evolution / Inhred Strain |
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| Research Abstract |
Human MHC (major histocompatibility complex) extending over 3.6 Mb at 6p21.3 contains many genes essential for adaptive immunity. The MHC is one of the most polymorphic regions of the human genome, and this polymorphism is believed to be formed by adaptive evolution against various pathogens. To clarify MHC polymorphism in bony fish, we determined the complete nucleotide sequence of the MHC class I region of the HNl strain (Northern Japan population-derived inbred medaka) and compared it with the published sequence of the Hd-rR strain (Southern Japan population-derived inbred medaka) MHC class I region. The compared region (〜 420 kb) was divided into three segments based on degree of sequence conservation. The middle segment (〜lOO kb) was so divergent between HNI and Hd-rR that it is almost impossible to make alignment, whereas the other two segments were easily aligned with an average 96.2% nucleotide identity between HNI and Hd-rR. The middle segment encompassed two MHC class I genes and immunoproteasome subunit genes, PSMB8 and PSMB10, and one class I gene and the PSMB8 gene were extremely divergent between two strains. This result indicates that the middle segment is evolving not only by simple nucleotide substitutions but also by extensive DNA rearrangement such as duplication, deletion and insertion within the medaka species.
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Research Products
(22 results)
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[Publications] Azumi, K., De Santis, R., De Tomaso, A., Rigoutsos, I., Yoshizaki, F., Pinto, M.R., Marino, R., Shida, K., Ikeda, I., Ikeda, M., Arai, M., Inoue, Y., Shimizu, T., Satoh, N., Rokhsar, D.S., Du Pasquier, L, Kasahara, M., Satake, M., Nonaka, M.: "Genomic Analysis of Immunity in a Urochordate and the Emergence of the Vertebrate Immune System"Waiting for Godot Immunogenetics. 55. 570-581 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohta, Y., Powis, S.J., Lohr, R.L., Nonaka, M., Du Pasquier, L., Flajnik, M.: "Two highly divergent ancient allelic lineages of the transporter associated with antigen processing (TAP) gene in Xenopus ; further evidence for co-evolution among MHC class I"Eur, J, Immunol.. 33. 3017-3027 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Nonaka, M.I., Hishikawa, Y., Moriyama, N., Koji, T., Ogata, R.T., Kudo, A., Kawakami, H., Nonaka, M.: "Complement C4b-binding protein as a novel murine epididymal secretory protein."Biol Reprod. 69. 1931-1939 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Terado, T., Okamura, K., Ohta, Y., Shin, D.H., Smith S.L., Hashimoto, K., Takemoto, T., Nonaka, M.I., Kimura, H., Flajnik, M.F., Nonaka, M.: "Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC."J. Immunol.. 171. 2461-2466 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Endoy, Y., Nonaka, M., Saiga, H., Kakinuma, Y., Matsushita, A., Takahashi, M., Matsushita, M., Fujita, T.: "Origin of mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 involved in the lectin complement pathway is tracted back to an invertebrate, amphioxus lineage."J. Immunol.. 170. 4701-4707 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Shida, K., Terajima, D., Uchino, R., Ikawa, S., Ikeda, M., Asano, K., Watanabe, T., Azumi, K., Nonaka, M., Satou, Y., Satoh, N., Satake, M., Kawazoe, Y., Kasuya, A.: "Hemocytes of Ciona intestinalis express multiple genes involved in innate immune host defense."Biochem. Biophys. Res. Commun.. 302. 207-218 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Dehal, P., Satou, Y., Campbell, R.K., Chapman, J., Degnan, B., De Tomaso, A., Davidson, B., Di Gregorio, A., Gelpke, M., Goodstein, D.M., Harafuji, N., Hastings, K.E., Ho, I., Hotta, K., Huang, W., Kawashima, T., Lemaire, P., Martinez, D., Meinertzhagen, I.A., Necula, S., Nonaka, M.: "The draft genome of Ciona intestinalis : insights into chordate and vertebrate origins."Science. 298. 2157-2167 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Matsuo, M.Y., Asakawa, S., Shimizu, Y., Kimura, H., Nonaka, M.: "Nucleotide sequence of the MHC class I genomic region of a teleost, the medaka (Oryzias latipes)."Immunogenetics. 53. 930-940 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Terado, T., Smith, S.L., Nakanishi, T., Nonaka, M.I., Kimura, H., Nonaka, M.: "Occurrence of strutural specialization of the serine protease domain of complement factor B at the emergence of jawed vertebrates and adaptive immunity."Immunogenetics. 53. 250254 (2001)
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[Publications] Nakao, M., Mutduro, J., Obo, R., Fujiki, K., Nonaka, M., Yano T.: "Molecular cloning and protein analysis of divergent forms of the complement complement component C3 from a bony fish, the common carp (Cyprinus carpio) : The presence of variants lacking the catalytic histidine."Eur. J. Immunol.. 30. 858-866 (2000)
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[Publications] Naruse, K., Fukamachi, S., Mitani, H., Kondo, M., Matsuoka, T., Kondo, S., Hanamura, N., Morita, Y., Hasegawa, K., Nishigaki, R., Shimada, A., Wada, H., Kusakabe, T, Suzuki, N., Kanamori, A., Terado, T., Kimura, H., Nonaka, M., Shima, A.: "A detailed linkage map of medaka, Oryzias latipes : Comparative genomics and genome evolution."Genetics. 154. 1773-1784 (2000)
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[Publications] Kuroda, N., Naruse, K., Shima, A., Nonaka, M., Sasaki, M., Nonaka, M.: "Molecular cloning and linkage analysis of complement C3 and C4 genes of the Japanese medaka fish."Immunogenetics. 51. 117-128 (2000)
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「研究成果報告書概要(欧文)」より
