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2001 Fiscal Year Final Research Report Summary

Studies on mechanisms of insecticide resistance and the application of characteristics of insecticide resistance for the integrated pest management

Research Project

Project/Area Number 11356002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 植物保護
Research InstitutionNagoya University

Principal Investigator

MIYATA Tadashi  Nagoya University, Graduate School of Bioagr. Sci., Professor, 大学院・生命農学研究科, 教授 (20023476)

Co-Investigator(Kenkyū-buntansha) NAGATA Toru  Ibaragi University, School of Agriculture, Professor, 農学部, 教授 (40301816)
TANAKA Toshiharu  Nagoya University, Graduate School of Bioagr. Sci., Associate Professor, 大学院・生命農学研究科, 助教授 (30227152)
KOBAYASHI Michihiro  Nagoya University, Graduate School of Bioagr. Sci., Professor, 大学院・生命農学研究科, 教授 (60111837)
MOTOYAMA Naoki  Chiba University, School of Horticulture, Professor, 園芸学部, 教授 (20124662)
KONO Yoshiaki  Tsukuba University, Institutee of Agriculture and Forestry, Professor, 農林学系, 教授 (10225386)
Project Period (FY) 1999 – 2001
KeywordsMechanisms of insecticide resistance / acetylcholineateraae / negatively correlated cross resistance / conservation of natural enemies / cytochrome P450 / monitoring for insecticide resistance / nucleopolyhedrovirus / greenrice leafhopper
Research Abstract

1 Clarification of mechanisms of insecticide resistance. Reduced sensitivity of AChE to insecticides which is one of the most important resistance factor. AChE cDNA are cloned and their aminoacids sequences were clarified in various insect pests such as Nephotetix cincticeps, Cidex tntaeniomychus, etc. Results indicated that insecticide resistance was caxised not only by the point mutation but also by the post translation By assaying AChE sensitivity to insecticides, combination of insecticides which show negatively con-elated cross resistance were screened. In OP resistant house fly, glutahtion-S-transferase activity is higher in males than in females the male germinating factor is located on the 2^<nd> chromosome, high GST activity in males were caused by ihe amplification of GST gene. Cytochrome P450 gene (CYP9A) which is responsible for permethnn resistance in Spodptera exigua was analyzed.
2. Establishment of the integrated pest management based oncharacteristics of insecticide resistant insects. Development of simple monitoring methods for insecticide resistance is one of the most important points for us establish a strategy for insecticide resistance. By assaying enzyme activities (esterase, AChE, GST) of individual insecte which an involved in insecticide resistance were developed (green rice leaf- and plant hoppers, house fly, rice water weevil, etc.). In case we could not apply biochemical monitoring methods, simple bioassay methods were developed against diamondback moth, western flower thrips, etc. Introduction of combination of insecticides which show negatively con-elated cross resistance, insecticide resistant natural enemies, conservation of natural enemies with selective insecticides, nucleopolyhedrovirus, ete. were also effective to reduce the selection pressure by insecticides and can contribute to delay or retard the development of insecticide resistance.

  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Shirata, N.: "Replication of Nucleopolyhedroviruses of Autograph californica (Lepidoptera : Noctuidae), Bombyx mori (Lepidoptera : Bombycidae), Hyphantria cunea (Lepidoptera : Arctiidae), and Spodoptera exigua (Lepidoptera : Noctuidae) in Four lepidopteran cell Lines"Appl. Entomol. Zool.. 34. 507-516 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, M.: "Monitoring of organophosphate and carbamate resistance and identifying a combination of insecticides showing negatively correlated cross resistance in the green rice leafhopper (Nephotettix cincticeps Uhler) (Hemiptera : Deltocephalidae)"Appl. Entomol. Zool.. 34. 525-530 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda, M.: "Cell-cycle perturbation in Sf9 cells infected with Autpgrapha californica nucelopolyhedrovirus"Virology. 258. 176-188 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, Y.: "Negatively correlated cross-resistance between N-methyl carbamate and monocrotophos in green rice leafhopper, Nephotettix cincticeps Uhler"J. Pesticide Sci.. 24. 364-368 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomita, T: "Absence of protein polymorphism attributable to insectiside-insensitivity of acetylcholinesterase in the green rice leafhopper, Nephotettix cincticeps"Insect Biochem. Mol. Biol.. 30. 325-333 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 呉福泉: "中国で分離したSpodoptera exigua(Lepidoptera : Nocutuidae)核多角体病ウイルスのクローニングと培養細胞における増殖特性"日蚕雑. 69. 177-189 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, M.: "The genotype and heredity of modified acetylcholinesterase of the green rice leafhopper (Nephotettix cincticeps Uhler)"Pestic. Biochem. Physiol.. 66. 73-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, M.E.: "Selection for resistance and susceptibility to methidathion and cross resistance in Amblyseius womersleyi Schicha (Acari : Phytoseiidae)"Appl. Entomol. Zool.. 35. 393-399 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ninsin, K.D.: "Decreased susceptibilities of four field populations of the diamondback moth, Plutella xylostella (L) (Lepidoptera : Yponomeutidae), to acetamiprid"Appl. Entomol. Zool.. 35. 591-595 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野哲: "ミカンキイロアザミウマから花をどう守るか"グリーンレポート. 338. 10-11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水川徹: "長距離移動性稲ウンカ類の殺虫剤抵抗性"植物防疫. 54. 54-59 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lavina, B.A.: "Biological characterization of a nucleopolyhedrovirus of Spodoptera litura (Lepidoptera : Nocutuidae) isolated from the Philippines"Biolocical Control. 20. 39-47 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotoshiro, H.: "Identification of noviral infection-specific polypeptides in the midgut of the silkworm, Bombyx pmori, infected with B. mori densovirus type 2"J. Insect Biotech. Sericol.. 70. 39-48 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda, M.: "Characterization of Autographa californica nucleopolyhedrovirus infection in cell lines from Bombyx mori"J. Insect Biotech. Sericol.. 70. 49-58 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, M.E.: "Methidathion resistance mechanisms in Amblyseius wormersleyi Schicha (Acari : Phytoseiidae)"Pestici. Biochem. Physiol.. 69. 1-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katou, Y.: "Characterization of Bombyx mori nucleopolyhedrovirus infection of Spodoptera frugiperda cells"J. Insect Biotec. Sericol.. 70. 137-147 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazaki, T.: "Fenitrothion insensitive acetylcholinesterases of the housefly, Musca domestica associated with point mutation"Insect Biochem. Mol. Biol.. 31. 991-997 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugiyama, S.: "Insecticidal activity and cuticular penetration of indoxacarb and its N-decarbomethoxylated metabolite in organophosphorus insecticide-resistant and -susceptible strains of the housefly, Musca domestica (L)"J. Pesticide Sci.. 26. 117-120 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, T.: "Polymorphism in the acetylcholinesterase gene of the housefly, Musca domestica L"Appl. Entomol. Zool.. 36. 377-380 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsurumachi, Y.: "Insecticidal activity of oxadiazine insecticide indoxacarb and its N-decarbomethoxylated metabolite and their modulations of voltage-gated sodium channels"Appl. Entomol. Zool.. 36. 381-385 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lavina-Caoili, B.A.: "Comparative in vitro analysis of geographic variants of nucleopoluhedrovirus of Spodoptera litura isolated from China and the Philippines"J. Insect Biotech. Sericol.. 70. 199-209 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takada, Y.: "Effects of various insecticides on the development of the egg parasitoid Trichogramma dendrolimi (Hymenoptera : Trichogrammatidae)"J. Econ. Entomol.. 94. 1340-1343 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野哲: "環境保全を意識したアブラナ科野菜のコナガ防除 〜被覆資材の活用と1剤1作のローテーションへ〜"園芸新知識. 7. 17-20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野義明: "アセチルコリンエステラーゼの感受性低下による殺虫剤抵抗性"植物防疫. 55. 316-320 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata, T.: "Inter-Country Forecasting System and Management for Brown Planthopper in East Asia"Rural Development Administration of Vietnam and FAO, Hanoi, Vietnam. 249 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyata, T.: "Management of Diamondback Moth and Other Crucifer Pests"Melbourne University Press, Melbourne, Australia (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirata, N.: "Replication of nucleopolyhedroviruses of Autograph californica (Lepidoptera : Noctuidae), Bomhyx mori (Lepidoptera : Bombycidue), Hyphyantrin cunea (Lepidoptera : Aretiidae), and Spodoptera exigua (Lepidoptera : Noctuidae) in four lepidopteran cell lines"Appl. Entomol.Zool. 34. 507-516 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, M.: "Monitoring of organophosphate and carbamate resistance and klentifying a combination of insecticides showing negatively correlated cross resistance in the green rico leafhoopper(Nephotettix cincticeps Uhler) (Hemiptera : Deltocephalidae)"Appl. Entomol. Zool.. 34. 525-530 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, M.: "Cell-cycle perturbation in S19 cells infected with autographa californica nucleopolyhedroviirus"Viroloogy. 258. 176-188 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Y.: "Negatively correlated cross-resistance between H-methyl carbamate and monocrotorhos in green rice leafhopper, Nephotettix cincticeps Uhler"J. Pesticide Sci.. 24. 364-368 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomita, T.: "Absence of protein polymorphism"Insect Biochem. Mol. Biol.. 30. 325-333 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wu, F. Q.: "Cloning and biological characterization of Spodoptera exigua mucleoplyhedroviruses isolated in"J. Insect Biotech. Sericol.. 69. 177-189 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, M.: "The genotype and geredity of modified acetylcholinesterase of the green rice leafhopper"Pestic. Biochem. Phyiol. G6. 73-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M. E.: "Selection for resistance and susceptibility to methidation and cross resistance in Amblyseitus womersleti Schicha(Acart phytoseiidae)"Appl. Entomol. Zonl.. 35. 393-399 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ninsin, K. D: "Decreased susveptibilities of four field populations of the diamndback moth, plutella xylosstella (L)(Lepidoptera : Yponomeutidae), to acetamiprid"Appl. Eutomol. Zool.. 35. 591-595 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono, S.: "How should we protect ornamental crops from the western flower thrips?"Green Report. No.338. 10-11 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagato, T.: "Insecticide resistance of long-range migrating rice planthoppers"Plant Protection. 54. 54-59 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lavina, B. A.: "Biological characterization of a nucleopolyhedrovirus of Spodoptera litura (Lepidoptera : Nocutuidae) isolated from the Phelippines"Biolocical Control. 20. 39-47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotoshiro, H.: "Identification of nonviral infection-specific polypeptides in the midgut of the silkworm, Bombyx pmori, infected with B. mori densovirus type 2"J. Insect Biotech. Sericol.. 70. 39-48 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, M.: "Characterization of Aautographa californica nucleopolyhedrovirus infection in cell lines from Bomhyx Mori"J. Insect Biotech. Sericol.. 70. 49-58 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M. E.: "Methidathion resistance mechanisms in Amblyseius womersleyi Schicha (Acari : Phytoseii)"Pestici Biochem. Physiol.. 60. 1-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katou, Y.: "Characterization of Bombyx mori nucleopolyhedrovirus infection of Spodoptera frugiperda cell"J. Insect Biotec. Sericol.. 70. 137-147 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, T.: "Fenitrothion insensitive acethloholinesterases of the housefly, Muscn domestica associated with point mutation"Insect Biochem. Mol. Biol.. 31. 891-997 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiyama, S.: "Insecticidal activity and cuticular penetration of indozacarb and its N-decarbomeihoxylated melaholite in organophosphorus insecticide-resistant and -susceptible strains of the housefly, Musca domestica(L)"J. Pesticide Sci.. 26. 117-120 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, T.: "Polymorphism in the acetylcholinesterase gene of the housefly. Musca domestica L"Appl. Entomol. Zool.. 96. 377-380 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsurumachi, Y.: "Insecticidal activity of oxidiazine insecticide indoxacarb and its N-decnibomethoxylated metabolite and their moddulations of voltage-gated sodium channels"Appl. Entomol. Zool.. 36. 381-385 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lavina-Caoili, B. A.: "Comparative in vitro analysis of geographic variants of nucleopoluhedrovirus of Spodoptera litura isolated from China and the Philippines"J. Insect Biotech. Sericol.. 70. 199-209 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takada, Y., S, Kawamura and T. Tanaka.: "(2001) Effects of various insecticides on the development of the egg parusitoid Trichogramma dendrolinu (Hymenoptera : Trichgrammatidae)"J. Econ. Entomol.. 94. 1340-1343 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono, S.: "Control of diamondback moth on crucifer crops based on enviromental conservaton"New Information on Horticulture. 7. 17-20 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono, Y.: "Insensitive acetycholinesterase in insecticide resistance"Plant Protectin. 55. 316-320 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, T.: "Recent status of insecticide resistance of long-distance migrating rice planthoppers monitored in Japan, China and Malaysia. In Inter-coutry Forecasting System and Management for Brown Planthopper in East Asis"Rural Development administration of Vietnam and FAO, Hanoi, Vietnam. 169-177 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, T.: "Effects of several insecticides on the larval parasite, Cotesia phitellae Kurdjumov, of diamondback moth. Phetella xylostella(L). In Management of Diamondback Moth and Other Crucifer Pests (Ed. by Peter Ridland)"Melbourne University Press, Melbourne, Australia. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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