2001 Fiscal Year Final Research Report Summary
Development of Fluorescent Reagents for Chirality Recognition Based on π-Electron Interaction
| Project/Area Number |
11440214
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAKAMURA Hiroshi Hokkaido University, Grad. School of Environ. Earth Sci., Prof., 大学院・地球環境科学研究科, 教授 (00117194)
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| Co-Investigator(Kenkyū-buntansha) |
MOROZUJNI Tatsuya Hokkaido University, Grad. School of Sci., Inst., 大学院・理学研究科, 助手 (50271713)
YOSHIDA Noboru Hokkaido University, Grad. School of Environ. Earth Sci., Asso. Prof., 大学院・地球環境科学研究科, 助教授 (00158461)
TANAKA Shunitz Hokkaido University, Grad. School of Environ. Earth Sci., Prof., 大学院・地球環境科学研究科, 教授 (30142194)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Molecular Recognition / Excimer / Fluorescence Spectrum / Crown Ethers |
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| Research Abstract |
Development of the reagent with pyrene moieties in the terminal of oligopeptide chain Amino acid[ was selected as a target guest of molecular recognition. A molecular design was carried out as follows: L-Phenylalanine or related molecules were introduced on 1,4-diaminobutane by amide bond. To this compound, naphthalene or pyrene moieties were also introduced. This reagent was expected to form a complex with guest molecule by the hydrogen bonds, and to show a fluorescence change was observed at the complex formation.
However, a little fluorescence change was observed at the complex formation with L- or D- phenylalanine esters.
On the other hand, the ^1H NMR spectra showed the chirality recognition for L- or D- phenylalanine esters.
2. Development of cyclodextrin base reagents
Cyclodextrin was selected as a molecular recognizing moiety, and naphthalene group was introduced on the cyclodextrin via methylene chain with several carbon atoms.
This compound (reagent) exhibits a change of fluorescence intensity depending on pH off the aqueous solution, and can be used for a pH sensor.
This reagent also shows a fluorescence change at the complex formation with guest molecules such as (-)borneol or 1-adamentanol. Induced circular Dichroism of this reagent by the guest molecules shows the usefulness for the chirality recognition.
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Research Products
(24 results)
