2001 Fiscal Year Final Research Report Summary
Multiple analysis of the pathogenic mechanism of an re-emerging infectious disease, neonatal TSS-like exanthematous disease and related diseases
| Project/Area Number |
11470072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo Woman's Medical University |
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Principal Investigator |
UCHIYAMA Takehiko Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (00050550)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI Junji Tokyo Women's Medical University, School of Medicine, Lecture Instructor, 医学部, 講師 (70182300)
IMANISHI Kenichi Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (20132920)
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| Project Period (FY) |
1999 – 2001
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| Keywords | TSS / TSST-1 / superantigen / Tcell / NTED / SDM |
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| Research Abstract |
In 1998 we have clarified the pathogenic mechanism of an infectious disease caused by a superantigenic toxin, and designated this disease neonatal TSS-like exanthematous disease (NTED). Under the current project, we examined following subjects.
1) T cell response in patients with NTED: TSST- 1-reactive T cells increased to 3-fold the control level in the acute phase and decreased to 20% of the control level 1-2 months later. TSST-1-reactive CD4+ T cells in expanded state are in an anergic state. The presence of IgG-type antibodies to TSST-1 prevented manifestation of clinical symptoms in MRSA neonatal carriers.
2) Responsibility of T cell in neonates: We obtained data suggesting that susceptibility of T cells in neonates to anergy induction to TSST-1 is caused by an inability of tyrosine-kinase Lck to make functional association with tyrosine-phosphatase CD45. 3) Superantigen-induced T cell response in mice: Using mice implanted with an osmotic pump filled with SEA, it was found that SEA-reactive CD4+ T cells exhibited protracted expansion and a memory-type response. 4) Discovery of a new type of superantigenic toxin: We found a new type of superantigenic toxin, Streptococcus dysgalactiae-derived mitogen which activates Yβ1 + and Vβ23+ T cells in association with MHC class II molecules on antigenpresenting cells.
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