2000 Fiscal Year Final Research Report Summary
Identification of the ligand recognized by a mAb specific for CD4^+CD8^+ thymocyte
| Project/Area Number |
11670325
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
FUKUI Yoshinori Medical Institute of Bioregulation, KYUSHU UNIVERSITY Associate Professor, 生体防御医学研究所, 助教授 (60243961)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIRASAWA Senji Medical Institute of Bioregulation, KYUSHU UNIVERSITY Research Associate, 生体防御医学研究所, 助手 (10253535)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | T cell differentiation / CD4^+CD8^+ thymocytes / TCR / MHC / self peptides / positive selection / Thy1 molecule |
|---|
| Research Abstract |
Although the diversity of αβ T cell receptors (TCRs) theoretically reaches 10^<15> by random rearrangement of five gene segments and random nucleotide addition, mature T cells express highly selected TCRs in that they exhibit tolerance to self-antigenic peptides and restriction by self-MHC molecules. This mainly results from two reciprocal selection processes, positive and negative selection, acting at CD4^+CD8^+ stage during T cell development in the thymus. With the use of the mAb specific for CD4^+CD8^+ thymocytes, we found that CD4^+CD8^+ thymocytes lacking TCR- MHC/peptide complex interaction specifically express multimerized Thy1 molecules. On the other hand, we developed several transgenic-knockout mouse lines expressing distinct peptides as a single species. By comparing T cell differentiation among these mice, we found positive selection is both specific and degenerate, dependending on the amino acid residues at TCR contacts of the selecting self-peptides.
|
Research Products
(10 results)
-
-
-
-
-
-
[Publications] Fukui, Y., Oono, T., Cabaniols, J.P., Nakao, K., Hirokawa, K., Inayoshi, A., Sanui, T., Kanellopoulos, J., Iwata, E., Noda, M., Katsuki, M., Kourilsky, P., Sasazuki, T.: "Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by its amino acid residure at a T cell receptor contact."Proc.Nat.Acad.Sci.USA.. 97 (25). 13760-13765 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Kawamura, K., Yamamura, T., Yokoyama, K., Chui, D.H., Fukui, Y., Sasazuki, T., Inoko, H., David, C.S., Tabira, T.: "HLA-DR2-restricted responses to proteolipid protein 95-116 peptide cause autoimmune encephalitis in transgenic mice."J.Clin.Invest.. 105 (7). 977-984 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Matsuki, N., Ogasawara, K., Takami, K., Namba, K., Takahashi, A., Fukui, Y., Sasazuki, T., Iwabuchi, K., Good, R.A., Onoe, K.: "Prevention of infection of influenza virus in DQ6 mice, a human model, by a peptide vaccine prepared according to the cassette theory."Vaccine. 17 (9-10). 1161-1168 (2000)
Description
「研究成果報告書概要(欧文)」より
-
-
