2000 Fiscal Year Final Research Report Summary
Enhancement of graft-vs-leukemia (GVL) effect by monocyte-derived dendritic cells following cord blood transplantation
| Project/Area Number |
11670755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Mie University |
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Principal Investigator |
AZUMA Eiichi Mie University, Hospital, Associate Professor, 医学部・附属病院, 助教授 (50211008)
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| Project Period (FY) |
1999 – 2000
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| Keywords | cord blood transplantation / graft-vs-leukemia (GVL) effect / dendritic cell |
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| Research Abstract |
In cord blood transplantation, graft-vs-host disease (GVHD) is mild and graft-vs-leukemia (GVL) effect may not take place. There are several cell types that may induce GVHD.Dendritic cells (DC) present allogeneic peptide to T cells that attack host. In this project, we examined whether DC may enhance allo-reaction, ie.GVHD/GVL.Since our preliminary data showed that DC could be induced from cord blood monocytes, we examined the characteristics of cord blood monocytes. Cord blood monocytes showed lower antigen-presentig capacity than adult blood, and functionally immature (Pediatr Int. in press. 2001), Next, we examined the mechanisms of T-cell apoptosis following antigen priming (Eur.J.Immunol. 2000), because T-cells primed with DC may apoptose early in the post-transplant. Then, we investigated T-cell recovery following allogeneic hematopoietic stem cell transplantation. We found that naive T-cell recovery is inversely correlated with donor age. These data suggest that T-cell recovery is prompt following cord blood transplantation (Brit.J.Haematol. 2000). Thus, T-cell following cord blood transplantation can be used to induce GVL effect with the help of DC.
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