2001 Fiscal Year Final Research Report Summary
Pathogenesis and etiology of cutaneous NK/T lymphoproliferative disorders
| Project/Area Number |
11670843
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Okayama University (2001)
Fukushima Medical University (1999-2000) |
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Principal Investigator |
IWATSUKI Keiji Okayama University Graduate School of Medicine and Dentistiry. Professor, 大学院・医歯学総合研究科, 教授 (80126797)
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| Co-Investigator(Kenkyū-buntansha) |
OONO Takashi Okayama University Hospital, Assistant, 医学部附属病院, 助手 (80203884)
FUJIMOTO Wataru Okayama University Hospital, Lecturer, 医学部附属病院, 講師 (50165429)
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| Project Period (FY) |
1999 – 2001
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| Keywords | NK / T cell lymphoma / EB virus / latent infection / mosquito hypersensitivity / hydroa vacciniforme |
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| Research Abstract |
In order to elucidate the EBV gene expression, we examined 8 patients with cutaneous EBV-related NK/T-cell lymphomas, including 6 patients with NK-cell phenotypes and 2 patients with T-cell phenotypes. All samples from the patients contained EBV nuclear antigen(EBNA)-1 mRNA which was transcribed using the Q promoter, whereas both the Q promoter and another upstream promoter (Cp/Wp) were used in 3 EBV-positive cell lines, B95.8, Raji and Jiyoye. Latent membrane protein-1 (LMP-1) mRNA was detected in 7 out of 8 patients and all cell lines, whereas EBNA-2 transcripts were found only in the cell lines. The BHRF1 (viral bcl-2 homologue) and BCRF1 (viral interleukin(vlL)-10) gene transcripts were detected in 1 (NK2) and 2 patients (NK2 and 3), respectively. The patient (NK2) with UK-cell lymphoma expressing the both transcripts died of rapid progression of the illness. No LMP-1, EBNA-2 or ZEBRA antigens were detected in the skin lesions by immunostaining although they were positive in the cell line cells.
One EBV-infected NK cellI line designated NOY-1 was established from a patient with hypersensitivity to mosquito bite associated with oligoclonal proliferation of EBV-infected NK cells in the blood. Clonal expansion of the cells was observed in the cutaneous lesions following mosquito bite. The cell line cells contained 7.5 copies of EBV-DNA, and produced high levels of IFN-gamma by stimulation.
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[Publications] Ito M, Ohira H, Miyata M, Suzuki T, Sato Y, Kaise S, Nishimaki T, Sakuma H, Nihei Y, Iwatsuki K, Kasukawa R: "Cytophagic histiocytic panniculitis improved by combined CHOP and cyclosporin A trealment"Intern Med. 38. 296-301 (1999)
Description
「研究成果報告書概要(和文)」より
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[Publications] M. Ito, H. Ohira, M. Miyata, T. Suzuki, Y. Sato, S. Kaise, T. Nishimaki, H. Sakuma, Y. Nihei, K. Iwatsuki, R. Kasukawa: "Cytophagic histiocytic panniculitis improved by combined CHOP and cyclosporin A treatment."Intern Med.. 38. 296-301 (1999)
Description
「研究成果報告書概要(欧文)」より
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