2000 Fiscal Year Final Research Report Summary
Establishment of new cold preservation method for intestinal graft
| Project/Area Number |
11671258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
YAGIHASHI Atsuhito Department of Clinical Laboratory Medicine, Sapporo Medical University Assistant Professor, 医学部, 講師 (40260757)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Naoki Department of Clinical Laboratory Medicine, Sapporo Medical University Professor, 医学部, 教授 (12217125)
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| Project Period (FY) |
1999 – 2000
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| Keywords | heat-shock protein / ischemia-reperfusion injury / cold presrvation / small intestine / IEC-18 |
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| Research Abstract |
In this study, we investigate whether the induction of hsp-70 could reduce small intestinal preservation injury in vitro. Intestinal epiethelial cell (IEC)-18 cells were divided two experimental groups. In group 1, the cells were thermally stressed and hsp-70 was induced. In group 2, the cells were cultured at 37℃ without thermal stress. Heat shock was performed in an oven at 43℃ for 30minutes. Cells were then placed in at 37℃ for 2 hours and then preserved at 4℃ in University of Wisconsin (UW) solution for 24 hours. In group 1, the cells were stained with anti-heat shock protein (hsp)-70 antibody 2 hours after thermal stress, but staining was weak in group 2. Magnitude of cold preservation injury was weak in group 1 than in group 2. Survival cell number after cold preservation was higher in group 1 than in group 2. LDH in culture medium 2 hours after cold preservation was higher in group 2 than in group 1. Apoptosis 24 hours after cold preservation was reduced in group 1 when compared with group 2.
These results suggest that after inducion of hsp-70 IEC cells become resistant to cold preservation injury, reducing apoptosis.
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Research Products
(8 results)
