2001 Fiscal Year Final Research Report Summary

Cryopresevation of Ovarian Tissue and organ culture

Research Project

Project/Area Number	11671640
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Yokohama City University
Principal Investigator	IKEDA Mario Yokohama City University, Dept. OB/GY, Lecturer, 医学部附属病院, 講師 (50254173)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Tsuneo Yokohama City University, Dept. OB/GY, Assistant Professor, 医学部附属病院, 助教授 (60179497)
Project Period (FY)	1999 – 2001
Keywords	cryopreservation / ovary / tissue / vitrification
Research Abstract	Mouse Germinal Vesicle (GV) stage oocytes and ovarian tissues were cryopreserved by vitrification. GV-oocytes or ovarian tissues were loaded in two freezing media (EFS 20 and EFS 40) or three freezing media (EPS 10, EFS 20 and EFS 40), respectively, and then stored in liquid nitrogen. GV-oocytes were morphologically survived (71.6 %). Morphologically frozen-thawed ovarian tissues have normal structure. After 17hr culture, Germinal Vesicle Breakdown (GVBD) was observed over 95 % of the frozen-thawed GV-oocytes or GV-oocytes isolated from frozen-thawed ovaries and then both oocytes progressed to Metaphase II (MII) stage (76.7 %, 73.6 %). MII oocytes matured from frozen-thawed GV-oocytes or ovarian GV-oocytes were competent to undergo fertilization and preimplantation development, but at a lower frequency than oocytes isolated from fresh ovaries (53.6 %, 56.1 % : 84.3 %).