2000 Fiscal Year Final Research Report Summary
Expression and role of exocytotic SNARE proteins on oocyte cortical reaction
| Project/Area Number |
11671649
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
KUJI Naoaki KEIO UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (80169987)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGAWA Kimio KYORIN UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (80129303)
YOSHIMURA Yasunori KEIO UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10129736)
TANAKA Hiroaki KEIO UNIVERSITY, SCHOOL OF MEDICINE, RESERACH ASSISTANT, 医学部, 助手 (40266565)
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| Project Period (FY) |
1999 – 2000
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| Keywords | oocytes / spermatozoa / testis / exocytosis / syntaxin / vesicle-associated membrane protein (VAMP) |
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| Research Abstract |
Exocytotic SNARE protein syntaxin expression was confirmed in both mouse oocytes and ovarian tissue, through SDS-PAGE using anti-ratsyntaxin 1B rabbit polyclonal antibody we raised previously. We also confirmed the existence of syntaxin 4 mRNA in total RNA from mouse oocytes by RT-PCR analysis, and cDNA sequence was completely coincides with mouse syntaxin 4.
Localization of this syntaxin 4 like molecule was examined by confocal microscopy, and it was revealed that syntaxin antigenesity was co-localized with cortical granule distribution before and after cortical reaction. After this observation, it was supposed syntaxin 4 might play some functional role during mouse oocyte cortical reaction as a component of fusion machinery.
Syntaxin was supposed to exist on acrosomal region of human spermatozoa form immunocytochemistry and SDS-PAGE analysis, suggested to engage sperm acrosome reaction.
Also, another exocytotic protein VAMP was expresseed on memmbrane fraction from rat testis. This VAMP-like protein was expressed on spermatogonia in testis before puberty in rat, although its functional role remained tobe elucidated.
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Research Products
(6 results)
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[Publications] Iwahashi K, Kuji N, Tanaka H, Kitaoka Y, Shiraishi S, Asada H, Hamatani T, Ozawa N, Miyazaki T, Sueoka K, Yoshimura Y, Akagawa K.: "Expression of HPC-1/syntaxin mRNA on murine oocytes during oocyte maturation and early embryo development."Jpn J Fertil Steril. 45 (4). 563 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka H, Kuji N, Tsuchiya S, Tanigaki R, Hamatani T, Ozawa N, Miyazaki T, Sueoka K, Yoshimura Y, Fujiwara T, Akagawa K.: "Expression of exocytotic SNARE protein on rat male germ cell lineage."Jpn J Fertil Steril. 45 (4). 568 (2000)
Description
「研究成果報告書概要(欧文)」より
