2002 Fiscal Year Final Research Report Summary
Evaluation of angiogenesis in AV-shunt loop and its use for tissue engineering
| Project/Area Number |
11671790
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
OHBA Sousuke Osaka Medical College Faculty of Medicine Assistant Professor, 医学部, 講師 (80233253)
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| Project Period (FY) |
1999 – 2002
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| Keywords | Tissue engineering / Basic fibroblast growth factor (bFGF or FGF-2) / Arterio-venous bundle / artificial dermis / Organ fabrication / vasa vasorum / chamber / microsurgery |
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| Research Abstract |
1. Comparison between AV shunt loop and AV bundle: which has more potential as a vascular carrier for an artificial skin flap? Results: Serial histological studies showed that new capillaries were derived not only from the 'vasa vasorum' of the femoral vessels but directly from the femoral vein in both AV-shunt and AV-bundle groups. Although the AV shunt loop showed a greater potential for producing new tissue and capillaries, the distal-ligation type of AV-bundle was thought to be a effective and practical vascular carrier for producing a tissue engineering skin flap.
2. Creation of a prefabricated artificial skin flap by the combined use of collagen sponge, bFGF and an AV-bundle in a chamber. The prefabricated artificial skin flap was created and could be transferred to the skin defect of a rabbit's ear microsurgically.
3. Creation of a larger and thinner prefabricated engineered skin flap by combined use of collagen sponge, FGF-2, and arterio-venous (AV) bundle in a porous rabbit chamber. Results: FGF-2 was useful for promoting large granulation tissue and a rich vascular network in the chamber. Creation of a prefabricated engineered skin flap was possible by adding a skin graft onto newly-formed granulation tissue in a chamber, and this could be transferred to the skin defect of a rabbit's ear microsurgically. Conclusion: The combined use of an existing AV bundle, FGF-1 and collagen sponge in the chamber is an alternative method for tissue (or organ) generation with its own nutrient vascular system.
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