2000 Fiscal Year Annual Research Report

アジアにおける難治性寄生虫病(エキノコックス症等)に関する免疫・分子疫学研究

Research Project

Project/Area Number	11694259
Research Institution	ASAHIKAWA MEDICAL COLLEGE
Principal Investigator	伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)
Co-Investigator(Kenkyū-buntansha)	岡本宗裕鳥取大学, 農学部・獣医学科・実験動物機能学, 助教授 (70177096) 堀井俊宏大阪大学, 微生物病研究所・分子原虫学, 教授 (80142305) 金澤保産業医科大学, 医学部・寄生虫学・熱帯医学, 教授 (10194888) 迫康仁旭川医科大学, 医学部・寄生虫学, 助手 (40312459) 中尾稔旭川医科大学, 医学部・寄生虫学, 助手 (70155670)
Keywords	新興・再興感染症 / エキノコックス症 / 多包虫症 / 単包虫症 / 有鉤嚢虫症 / 術前鑑別血清診断法 / ミトコンドリアDNA / 遺伝子診断法
Research Abstract	本研究成果の概要は下記の5項目としてまとめられる。 1.多包虫症、単包虫症、有鉤嚢虫症についての血清学的鑑別診断法の確立:基本的には世界で最も信頼性が高い血清診断法をそれぞれに対して確立している。多包虫症は1999年4月から施行されている新しい感染症予防法の下で、届け出が義務づけられた第4類感染症に含まれているが、外科的摘出病巣から多包虫病巣を確認する、術後診断に基づいている。旭川医大方式により術前確定血清診断が可能な現在、術前確定診断無しの外科的処置だけは避けなければならない。誤診例が一例もなく、国際的にも高く評価されている旭川医大方式を標準検査法として普及させるべき時期であると提案したい。2.遺伝子組換え抗原を用いる診断法についても基礎的研究はほぼ完了している。今後はキット化に向けた研究が中心になる。3.疫学調査への展開:中国(多包虫症、単包虫症、嚢虫症)、インドネシア(嚢虫症)、アフリカ(タンザニア、モザンビーク)(嚢虫症)、アメリカ(メキシコ、エクアドル)(嚢虫症)を中心に、エキノコックス症、嚢虫症に関する調査研究、技術指導に参加した。WHOを通して、ベトナムにおける嚢虫症、単包虫症対策への協力要請が来ている。4.多包条虫、有鉤条虫それぞれのミトコンドリアDNA全塩基配列の解読に世界で最初に成功し、分子生物学的基礎研究(条虫によるアミノ酸情報の読み始め、読み終えコドンのユニークな利用)から分子疫学(世界各地における寄生虫の適応拡散、病原性の多様性等)への応用展開が可能になった。5.1999年ならびに2001年のエキノコックス症国際会議、2000年のNATO Advanced Research Workshop、バンコックで開催された人畜共通寄生虫病会議等でこれらの寄生虫病に関する血清診断法の進展について招聘講演、シンポジスト、座長を引き受けてきている。

Research Products

(38 results)

All Other

All Publications (38 results)

[Publications] Ito A et al.: "Introduction to the Symposium on Cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Margono SS et al.: "Cysticercosis in Indonesia : epidemiological aspects"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "NOD-scid mouse as an experimental animal model for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Okamoto M et al.: "Molecular variation of Taenia solium in the world"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Sako Y et al.: "Recent advances in serodiagnosis for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).
[Publications] Ito A et al.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 32(in press).
[Publications] Margono SS et al.: "The problem of taeniasis and cysticercosis in Irian Jaya (Papua), Indonesia"Proceedings of the 6th Asian-Pacific Congress for Parasitic Zoonoses. 55. 64 (2000)
[Publications] Ito A et al.: "Comparison of antigenicity of protoscolex and micro vesicle of Echinococcus multilocularis prepared from rats"Journal of Helminthology. (in press).
[Publications] Wandra T et al.: "Seroepidemiology of neurocysticercosis in Irian Jaya (West Papua), Indonesia in 1996-1997 and Papua New Guinea in 1997"Acta Tropica. 78(in press).
[Publications] Subahar R et al.: "Taenia solium infection in Irian Jaya (West Papua), Indonesia : A pilot serological survey of human and porcine cysticercosis in Jayawijaya District"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).
[Publications] Nakao M et al.: "The complete nucleotide sequence of the cestode Echinococcus multilocularis mitochondrial genome"Genetics. (in press).
[Publications] Jiang L et al.: "Immunodiagnostic differentiation for alveolar and cystic echinococcosis using ELISA test with 18kDa antigen extracted from Echinococcus protoscoleces"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).
[Publications] Craig PS et al.: "An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China"Acta Tropica. 77. 167-177 (2000)
[Publications] Nakao M et al.: "Mitochondrial genetic code in cestodes"Molecular and Biochemical Parasitology. 111. 415-424 (2000)
[Publications] Sako Y et al.: "Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes"Journal of Clinical Microbiology. 38. 4439-4444 (2000)
[Publications] Wang H et al.: "An epidemiological survey on human hydatidosis in southern Qinghai platau"Chinese Journal of Parasitic Disease Control. 13. 37-41 (2000)
[Publications] Wang H et al.: "Infections of larval and adult Echinococcus multilocularis in human and animals in Qinghai province"Chinese Journal of Parasitic Disease Control. 13. 120-123 (2000)
[Publications] Qiu JM et al.: "Epidemiological study on human hydatidosis in Tibetan region of western Sichuan"Chinese Journal of Zoonoses. 16. 77-80 (2000)
[Publications] Wandra,T et al.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya,Irian Jaya, Indonesia, 1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 46-50 (2000)
[Publications] Ito,A et al.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticerosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)
[Publications] Qiu,Jm et al.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)
[Publications] Ito,A: "Differential serodiagnosis for larval cestode infections : cystic echinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)
[Publications] Kimura,M et al.: "Cystic echinococcosis in a Jordanian patient : albendazole in ashort-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)
[Publications] Wang,IC et al.: "Oncospheres of Taenia solium and T.saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)
[Publications] Willingham,III AL et al.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)
[Publications] Ito,A et al.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)
[Publications] Ohsaki,Y et al.: "Neurocysticercosis without detectable specific antibody"Internal Medicine. 38. 67-70 (1999)
[Publications] Ito,A et al.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)
[Publications] Ito,A et al.: "Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E.multilocularis protoscolex (Em18)"American Journal of Tropical Medicine and Hygiene. 60. 188-192 (1999)
[Publications] 伊藤亮: "エキノコックス症-世界・日本での現状、診断・治療・予防-"北海道プライマリー・ケア研究会会報. 19. 29-39. (2001)
[Publications] 伊藤亮: "旭川医科大学におけるエキノコックス症研究の現状と日本におけるエキノコックス症の問題点."北海道医学雑誌. 76. 3-8 (2001)
[Publications] Ito A et al.: "Mitochondrial DNA of Taenia solium : from basic to applied science"Taenia solium Cysticercosis CAB International (UK) Prabhakar S, Singh G (in press).
[Publications] Ito A et al.: "Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS, Pawlowski ZS (in press).
[Publications] Ito A et al.: "Neurocysticercosis in A sia : serology/seroepidemiology in humans and pigs"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS, Pawlowski ZS (in press).
[Publications] 伊藤亮: "シンプル微生物学(改訂第3版)第9編原虫学."南江堂. (2000)
[Publications] 伊藤亮: "条虫感染における免疫(2)組織寄生(幼条虫)日本における寄生虫学の研究第7巻6章"(1999)
[Publications] 辻守康,近藤力王至,伊藤亮: "寄生蠕虫症の免疫診断日本における寄生虫学の研究第6巻5章"(1999)

2000 Fiscal Year Annual Research Report

アジアにおける難治性寄生虫病(エキノコックス症等)に関する免疫・分子疫学研究

Principal Investigator

伊藤 亮 旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

Research Products

[Publications] Ito A et al.: "Introduction to the Symposium on Cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Margono SS et al.: "Cysticercosis in Indonesia : epidemiological aspects"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "NOD-scid mouse as an experimental animal model for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Okamoto M et al.: "Molecular variation of Taenia solium in the world"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Sako Y et al.: "Recent advances in serodiagnosis for cysticercosis"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 33(in press).

[Publications] Ito A et al.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health (suppl). 32(in press).

[Publications] Margono SS et al.: "The problem of taeniasis and cysticercosis in Irian Jaya (Papua), Indonesia"Proceedings of the 6th Asian-Pacific Congress for Parasitic Zoonoses. 55. 64 (2000)

[Publications] Ito A et al.: "Comparison of antigenicity of protoscolex and micro vesicle of Echinococcus multilocularis prepared from rats"Journal of Helminthology. (in press).

[Publications] Wandra T et al.: "Seroepidemiology of neurocysticercosis in Irian Jaya (West Papua), Indonesia in 1996-1997 and Papua New Guinea in 1997"Acta Tropica. 78(in press).

[Publications] Subahar R et al.: "Taenia solium infection in Irian Jaya (West Papua), Indonesia : A pilot serological survey of human and porcine cysticercosis in Jayawijaya District"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).

[Publications] Nakao M et al.: "The complete nucleotide sequence of the cestode Echinococcus multilocularis mitochondrial genome"Genetics. (in press).

[Publications] Jiang L et al.: "Immunodiagnostic differentiation for alveolar and cystic echinococcosis using ELISA test with 18kDa antigen extracted from Echinococcus protoscoleces"Transactions of the Royal Society of Tropical Medicine and Hygiene. 95(in press).

[Publications] Craig PS et al.: "An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China"Acta Tropica. 77. 167-177 (2000)

[Publications] Nakao M et al.: "Mitochondrial genetic code in cestodes"Molecular and Biochemical Parasitology. 111. 415-424 (2000)

[Publications] Sako Y et al.: "Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes"Journal of Clinical Microbiology. 38. 4439-4444 (2000)

[Publications] Wang H et al.: "An epidemiological survey on human hydatidosis in southern Qinghai platau"Chinese Journal of Parasitic Disease Control. 13. 37-41 (2000)

[Publications] Wang H et al.: "Infections of larval and adult Echinococcus multilocularis in human and animals in Qinghai province"Chinese Journal of Parasitic Disease Control. 13. 120-123 (2000)

[Publications] Qiu JM et al.: "Epidemiological study on human hydatidosis in Tibetan region of western Sichuan"Chinese Journal of Zoonoses. 16. 77-80 (2000)

[Publications] Wandra,T et al.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya,Irian Jaya, Indonesia, 1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 46-50 (2000)

[Publications] Ito,A et al.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticerosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)

[Publications] Qiu,Jm et al.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)

[Publications] Ito,A: "Differential serodiagnosis for larval cestode infections : cystic echinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)

[Publications] Kimura,M et al.: "Cystic echinococcosis in a Jordanian patient : albendazole in ashort-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)

[Publications] Wang,IC et al.: "Oncospheres of Taenia solium and T.saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)

[Publications] Willingham,III AL et al.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)

[Publications] Ito,A et al.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)

[Publications] Ohsaki,Y et al.: "Neurocysticercosis without detectable specific antibody"Internal Medicine. 38. 67-70 (1999)

[Publications] Ito,A et al.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)

[Publications] Ito,A et al.: "Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E.multilocularis protoscolex (Em18)"American Journal of Tropical Medicine and Hygiene. 60. 188-192 (1999)

[Publications] 伊藤亮: "エキノコックス症-世界・日本での現状、診断・治療・予防-"北海道プライマリー・ケア研究会会報. 19. 29-39. (2001)

[Publications] 伊藤亮: "旭川医科大学におけるエキノコックス症研究の現状と日本におけるエキノコックス症の問題点."北海道医学雑誌. 76. 3-8 (2001)

[Publications] Ito A et al.: "Mitochondrial DNA of Taenia solium : from basic to applied science"Taenia solium Cysticercosis CAB International (UK) Prabhakar S, Singh G (in press).

[Publications] Ito A et al.: "Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS, Pawlowski ZS (in press).

[Publications] Ito A et al.: "Neurocysticercosis in A sia : serology/seroepidemiology in humans and pigs"Cestode Zoonoses : an Emergent and Global Problem IOS Press (Netherlands) Craig PS, Pawlowski ZS (in press).

[Publications] 伊藤亮: "シンプル微生物学(改訂第3版)第9編 原虫学."南江堂. (2000)

[Publications] 伊藤亮: "条虫感染における免疫(2)組織寄生(幼条虫)日本における寄生虫学の研究第7巻6章"(1999)

[Publications] 辻守康,近藤力王至,伊藤亮: "寄生蠕虫症の免疫診断日本における寄生虫学の研究第6巻5章"(1999)

伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

[Publications] 伊藤亮: "シンプル微生物学(改訂第3版)第9編原虫学."南江堂. (2000)