2000 Fiscal Year Final Research Report Summary
Development of Multiple Quantum NMR Spectroscopy and Imgaing : Imaging Application on Bound Water in Biological Systems.
| Project/Area Number |
11694305
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SEO Yoshiteru Dept. of Physiology, Kyoto Prefectural University of Medicine, Lecturer, 医学部, 講師 (90179317)
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| Co-Investigator(Kenkyū-buntansha) |
KUSAKA Yoshiaki Dept. of Orthopedic Surgery, Kyoto Prefectural University of Medicine, Lecturer, 医学部, 講師 (40205068)
SHINAR Hadassah Dept. of Chemistry, Tel-Aviv Univ., Lecturer
NAVON Gil Dept. of Chemistry, Tel-Aviv Univ., Professor
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| Project Period (FY) |
1999 – 2000
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| Keywords | Water / Dipole interaction / Quadrupolar interaction / Multiple-quantum NMR / DQF MRI / DQF CSI / Achilles tendon / Cartilage |
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| Research Abstract |
The Aims of this project is development of new functional MR imaging based on the multiple quantum NMR and the spectroscopic imaging to follow "order" structure of water and to use it to understand physiological processes in healthy and pathological tissues.
1) Development of double-quantum-filter NMR imaging : We have introduced H-1 slice selective double-quantum-filter NMR imaging (H-1 DQF MRI) which depicted ordered water (bound water) in biological tissues selectively, and also developed H-1 and H-2 spectroscopic double-quantum-filter NMR imaging (H-1 and H-2 DQF CSI) which depict water in a certain molecular environment through selective window of molecular dynamics.
2) Development of variable-angle loaded-cell H-2/H-1 probe : In order to visualize macroscopic order of the bound water, it is necessary to measure relaxation time and residual quadrapoler (or dipoler) splitting in various angle against the static magnetic field. We have built the world-first variable-angle loaded-cell probe for H-2/H-1 NMR, which can apply a static load up to 0.75 MPa to the cartilage sample with 1.0 cm in diameter.
3) Visualization of collagen structure in the articular cartilage by H-2/H-1 DQF CSI : In the intact bovine articular cartilage, we can depict the collagen structures in the superficial, tangenital, deep and carcified zones, Observation and analysis on the deformation of collagen structures under static loading are under going, and might be finished in 2001.
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[Publications] Seo, Y., Ikoma, K., Takamiya, H., Kusaka, Y., Tsoref, L., Eliav, U., Shinar, H.& Navon, G.: "^1H double-quantum-filtered MR imaging as a new tool for assessment of healing of the ruptured Achilles tendon."Magn.Reson.Med.. 42. 884-889 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Takamiya, H., Kusaka, Y., Seo, Y., Noguchi, M., Ikoma, K., Morimoto, T.& Hirasawa, Y.: "Characteristics of proton NMR T_2 relaxation of water in the normal and regenerating tendon."Jpn.J.Physiol.. 50 (6). 569-576 (2000)
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[Publications] Sogami, M., Era, S., Murakami, M., Seo, Y., Watari, H.& Uyesaka, N.: "Application of the transition state theory to water transport across cell membrane."Biochi.Biophys.Acta. (in press). (2001)
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[Publications] Kusaka, Y., Nakajima, S., Uemura, O., Aoshiba, H., Seo, Y.& Hirasawa, Y.: "Intradiscal solid phase displacement as a determinant of the centripetal fluid shift in the loaded intervertebral disc."Spine. 26 (in press). (2001)
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