The mechanism of neuropatic pain model

2000 Fiscal Year Final Research Report Summary

• Project/Area Number: 11694327
• Research Category: Grant-in-Aid for Scientific Research (B).
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: HYOGO COLLEGE OF MEDICINE
• Principal Investigator: NOGUCHI Koichi HYOGO COLLEGE OF MEDICINE, PROFESSOR, 医学部, 教授 (10212127)
• Co-Investigator(Kenkyū-buntansha): MIKI Kenji HYOGO COLLEGE OF MEDICINE, RESEARCH ASSOCIATE, 医学部, 助手 (60268555) ; KONDO Eiji HYOGO COLLEGE OF MEDICINE, ASSISTANT PROF., 医学部, 講師 (50273636) ; TOKUNAGA Atsushi HYOGO COLLEGE OF MEDICINE, ASSISTANT PROF., 医学部, 講師 (70254521) ; TSUBOI Yoshiyuki SCHOOL OF DENTISTRY NIHON UNIVERSITY, RESEARCH ASSOCIATE, 歯学部, 助手 (50246906) ; IWATA Koichi OSAKA UNIVERSITY, FACULTY OF DENTISTRY, ASSOCIATE PROF., 歯学部, 助教授 (60160115)
• Project Period (FY): 1999 – 2000
• Keywords: NEUROPATHIC PAIN / ELECTROPHYSIOLOGY / NERVE INJURY / C-FOS / ATF3 / 神経損傷 / 遺伝子発現

Research Abstract

We reported dorsal column-thalamic pathway involvement on thalamic hyperexcitability following peripheral nerve injury usin electrophysiology and neuropathic pain model. We also reported the activating transcription factor-3 induction by axotomy in sensory and motoneurons as a novel neuronal marker of nerve injury. We also reported the excitability of spinal cord and gracile nucleus neurons in rats with chronically injured sciatic nerve using c-fos expression. We also reported the differential effect of brain-derived neurotrophic factor on high-threshold mechanosensitivity in a rat neuropathic pain model. We also reported the differential regulation of P2X3 mRNA expression by peripheral nerve injury in intact and injured neurons in the rat sensory ganglia.

Research Products

• [Publications] Fukuoka.T., et.al.: "Differential regulation of alpha-and beta-CGRP mRNAs within oculomotor, trochlear, abducens, and trigeminal motoneurons in response to axotomy."Mol.Brain Res.. 63. 304-315 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tokunaga.A., et.al.: "Excitability of spinal cord and gracile nucleus neurons in rats with chronically-injured sciatic nerve examined by c-fos expression."Brain Res.. 847. 321-331 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tsujino.H., et.al.: "Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons : A novel neuronal marker of nerve injury."Mol.Cell.Neurosci.. 15. 170-182 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miki.K.,et.al.: "Differential effect of brain-derived neurotrophic factor on high-threshold mechanosensitivity in a rat neuropathic pain model."Neurosci.Lett.. 278. 85-88 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miki.K., et.al.: "Dorsal column-thalamic pathway is involved in thalamic hyperexcitability following peripheral nerve injury : a lesion study in rats with experimental mononeuropathy."Pain. 85. 263-271 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tuzuki,K., et.al.: "Differential regulation of P2X3 mRNA expression by peripheral nerve injury in intact and injured neurons in the rat sensory ganglia."Pain. (印刷中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukuoka, T., Tokunaga, A., Kondo, E., Miki, K., Tachibana, T.and Noguchi, K.: "Differential regulation of alpha- and beta CGRP mRNAs within oculomotor, trochlear, abducens, and trigeminal motoneurons in response to axotomy."Mol.Brain Res.. 63. 304-315 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yasuda T., Iwamoto T., Ohara M., Sato S., Kohri H., Noguchi K and Senba E.: "The novel analgesic compound OT-7100 (5-n-Butyl-7-(3, 4, 5-trimethoxybenzoylamino) pyrazolo [1, 5-a] pyrimidine) attenuates mechanical nociceptive responses in animal models of acute and peripheral neuropathic hyperalgesia."Jpn.J.Pharmacol. 79. 65-73 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Atushi Tokunaga, Eiji Kondo, Tetsuo Fukuoka, Kenji Miki, Yi Dai, Hiroaki Tsujino, Koichi Noguchi: "Excitability of spinal cord and gracile nucleus neurons in rats with chronically injured sciatic nerve examined by c-fos expression."Brain Res.. 847. 321-331 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tsujino.H., Kondo.E., Fukuoka.T., Dai.Y., Tokunaga.A., Miki.K., Yonenobu.K., Ochi.T.and Noguchi.K.: "Activating transcription factor 3 (ATF3) induction by axotomy in sensory and motoneurons : A novel neuronal marker of nerve injury."Mol.Cell.Neurosci.. 15. 170-182 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Dai.Y., Miki.K., Fukuoka.T., Tokunaga.A., Tachibana.T., Kondo.E.and Noguchi.K.: "Suppression of neuropeptides' mRNA expression by herbal medicines in a rat model of peripheral inflammation."Life Sci.. 66. 19-29 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miki.K., Fukuoka.T., Tokunaga.A., Kondo.E., Dai.Y.and Noguchi.K.: "Differential effect of brain-derived neurotrophic factor on high-threshold mechanosensitivity in a rat neuropathic pain model."Neurosci.Lett.. 278. 85-88 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miki.K., Iwata.K., Tsuboi.Y., Morimoto.T., Kondo.E., Dai Y., Ren.K.and Noguchi.K.: "Dorsal column-thalamic pathwa is involved in thalamic hyperexcitability following peripheral nerve injury : a lesion study in rats with experimental mononeuropathy."Pain. 85. 263-271 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yasuno.H., Fukazawa.K., Fukuoka.T., Kondo.E., Sakagami.M and Noguchi.K.: "Nerve growth factor applied onto the olfactory epithelium alleviates degenerative changes of the olfactory receptor neurons following axotomy."Brain Res.. 887. 53-62 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kenzo Tsuzuki, Eiji Kondo, Tetsuo Fukuoka, Dai Yi, Hiroaki Tsujino, Masafumi Sakagami and Koichi Noguchi: "Differential regulation of P2X3 mRNA expression by peripheral nerve injury in intact and injured neurons in the rat sensory ganglia"Pain. (In Press). (2001)
  • Description: 「研究成果報告書概要(欧文)」より