2000 Fiscal Year Final Research Report Summary
A new detection system for artificial synthetic steroid like compound using KO mouse lacking estrogen.
| Project/Area Number |
11839016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Kochi Medical School |
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Principal Investigator |
SHIZUTA Yutaka Kochi Medical School, Dept.Medical Chemistry, Professor, 医学部, 教授 (50025631)
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| Co-Investigator(Kenkyū-buntansha) |
KUGE Hideaki Kochi Medical School, Dept.Medical Chemistry, Research Associate, 医学部, 助手 (70304673)
TODA Katsumi Kochi Medical School, Dept.Medical Chemistry, Adjunct Professor, 医学部, 助教授 (40197893)
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| Project Period (FY) |
1999 – 2000
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| Keywords | estrogen / biohazard estrogen / a new assay method / KO mouse |
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| Research Abstract |
To establish a new detection system for harmful estrogen like chemical compounds. KO mouse lacking estrogen have been prepared. This female mouse becomes infertile due to immature ovary and uterus and they decay after growth but at becomes fertile when the mouse is supplement with estrogen directly birth. Many kind of estrogen analogs competitive action of estrogens to the mouse again those organs. The male mouse is also fertile due to abnormal sexual behavior, but it becomes fertile after supplemented with estrogen. It also lacks aggression against other male mouse. This is reversed by supplementation with estrogen, and the action of estrogen is inhibited by many estrogens analogs. Thus, KO mouse lacking estrogen prepared in this study is useful for bioassay of biohazard estrogen like chemical substances. We have also developed chemical assay method for harmful estrogen like stubstances in the brain of the mouse before and after estrogen treatment.
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[Publications] Shinoda, E., Yui, Y., Hattori, R., Tanaka, M., Inoue, R., Aoyama, T., Takimoto, Y., Mitsui, Y., Miyahara, K., Shizuta, Y., and Sasayama, S.: "Tissue Factor Pathway Inhibitor-2 Is a Novel Mitogen for Vascular Smooth Muscle Cells."J.Biol.Chem.. 274. 5379-5384 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Toda, K., Okada, S., Nakamura, K., Nishihara, M., Yokotani K., and Shizuta, Y.: "Concentrations of Monoamines and Acetylcoline in the Brains of Mice Lacking the Aromatase Cytochrome P450 Gene.""Molecular Steroidogenesis" (eds.Okamoto, M., Ishimura, Y., and Newada, H.). 141-143 (1999)
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[Publications] Nemoto, Y., Toda, K., Ono, M., Fujikawa-Adachi, K., Saibara, T., Onishi, S., Enzan, H., Okada, T., and Shizuta, Y.: "Altered Expression of Fatty Acid-Metabolizing Enzymes in Aromatase-Deficient Mice."J.Clin.Invest.. 105. 1819-1825 (2000)
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「研究成果報告書概要(欧文)」より
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